Regulation of mdm2 function
First Claim
Patent Images
1. An mdm2 gene targeting vector, comprising:
- (a) a first targeting sequence substantially identical to a DNA sequence 5′
of one or more exons of the mdm2 gene;
(b) a first recombinase recognition sequence;
(c) a second targeting sequence substantially identical to a DNA sequence of one or more exons of the mdm2 gene;
(d) a second recombinase recognition sequence; and
(e) a third targeting sequence substantially identical to a DNA sequence 3′
of one or more exons of the mdm2 gene.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention provides non-human transgenic animals having conditional mdm2 transgenes integrated into their genome, cells derived therefrom, and methods for making and using both.
13 Citations
29 Claims
-
1. An mdm2 gene targeting vector, comprising:
-
(a) a first targeting sequence substantially identical to a DNA sequence 5′
of one or more exons of the mdm2 gene;
(b) a first recombinase recognition sequence;
(c) a second targeting sequence substantially identical to a DNA sequence of one or more exons of the mdm2 gene;
(d) a second recombinase recognition sequence; and
(e) a third targeting sequence substantially identical to a DNA sequence 3′
of one or more exons of the mdm2 gene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
-
14. A transgenic non-human mammal whose somatic and germ cells comprise a conditional mdm2 allele having a first recombinase recognition sequence 5′
- of at least one exon of an mdm2 gene and a second recombinase recognition sequence 3′
of at least one exon of the mdm2 sequence, wherein at least some cells of the mammal exhibit decreased mdm2 expression upon exposure to a recombinase. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
- of at least one exon of an mdm2 gene and a second recombinase recognition sequence 3′
Specification