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Biosensor and method

  • US 20040217019A1
  • Filed: 06/04/2004
  • Published: 11/04/2004
  • Est. Priority Date: 07/31/2001
  • Status: Active Grant
First Claim
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1. A method of using an electrode strip for determining the concentration of creatinine, said electrode strip having a first working electrode, a second working electrode and a reference electrode wherein said first working electrode contains a first enzyme capable of catalyzing a reaction involving a substrate for creatine and said second working electrode contains a second enzyme capable of catalyzing a reaction involving a substrate for creatinine, said first working electrode, said second working electrode and said reference electrode being disposed in a fluid sample channel for measuring a fluid sample, said method comprising:

  • disposing said fluid sample into said channel of said electrode strip;

    applying a potential between said reference electrode and said first working electrode;

    measuring a first current generated between said first working electrode and said reference electrode and correlating said first current to a concentration of said creatine in said fluid sample;

    applying a potential between said reference electrode and said second working electrode;

    measuring a second current generated between said second working electrode and said reference electrode;

    subtracting said first current from said second current and obtaining a current difference whereby said current difference is representative of the concentration of creatinine in said fluid sample.

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