Alcohol product processes

• US 20040219649A1
• Filed: 03/10/2004
• Published: 11/04/2004
• Est. Priority Date: 03/10/2003
• Status: Abandoned Application

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Pedersen, Sven, Olsen, Hans Sejr, Festersen, Rikke Monica
• Application Number:
  US10/797,393
• Publication Number:
  US 20040219649A1
• Time in Patent Office: Days
• US Class Current: 435/161
• CPC Class Codes: C12C 5/004 Enzymes
• CPC Class Codes: C12C 7/04 Preparation or treatment of the mash
• CPC Class Codes: C12N 9/2411 Amylases
• CPC Class Codes: C12N 9/2414 Alpha-amylase (3.2.1.1.)
• CPC Class Codes: C12N 9/2417 from microbiological source
• CPC Class Codes: C12N 9/242 Fungal source
• CPC Class Codes: C12N 9/2425 Beta-amylase (3.2.1.2)
• CPC Class Codes: C12N 9/2428 Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12Y 302/01001 Alpha-amylase (3.2.1.1)
• CPC Class Codes: C12Y 302/01003 Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention relates to processes for production of an alcohol product from granular starch comprising a pre-treatment at an elevated temperature below the initial gelatinization temperature of said granular starch followed by simultaneous saccharification and fermentation, and optionally recovery of ethanol.

Claims

1-37. -37 (Canceled)

38. A process for production of an alcohol product comprising the sequential steps of:

(a) providing a slurry comprising water and granular starch,

(b) holding said slurry in the presence of an acid alpha-amylase and a glucoamylase at a temperature of 0°

C. to 20°

C. below the initial gelatinization temperature of said granular starch for a period of 5 minutes to 12 hours,

(c) holding said slurry in the presence of an acid alpha-amylase and a glucoamylase and a yeast at a temperature between 10°

C. and 35°

C. to produce ethanol and,

(d) optionally recovering the ethanol.

90. An enzyme composition comprising acid alpha-amylase activity and glucoamylase activity in a ratio of between 0.30 and 5.00 AFAU/AGU and one or more additional enzymes selected from the group consisting of cellulase, xylanase and phytase.

91. A mashing process comprising treating a mash with an acid alpha-amylase.

92. The process of claim 91, wherein the acid alpha-amylase is derived from a fungus.

93. The process of claim 91, wherein the acid alpha-amylase is derived from Aspergillus.

94. The process of claim 91, wherein the acid alpha-amylase is derived from A. niger.

95. The process of claim 91, wherein the acid alpha-amylase has the amino acid sequence shown in SEQ ID NO:

1

96. The process of claim 91, comprising;

(a) forming a mash comprising between 5% and 100% barley malt (w/w of the grist);

(b) prior to, during or after a) adding an acid alpha-amylase and at least one enzyme selected from the list comprising;

a protease, cellulase and a maltose generating enzyme.

(c) attaining within 15 minutes of a) an initial incubation temperature of at least 70°

C.;

(d) following c) incubating the mash at a temperature of at least 70°

C. for a period of time sufficient to achieve an extract recovery of at least 80%.