High sensitivity detection of and manipulation of biomolecules and cells with magnetic particles
First Claim
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1. A method of identifying the presence of magnetically labeled cell in a sample comprising the steps of:
- providing a sample containing one or more cells;
contacting the target cell with a magnetic label under conditions which permit the formation of a cell-label complex;
subjecting said sample to an applied magnetic field; and
detecting a characteristic response of said sample to an applied magnetic field.
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Abstract
The present invention generally relates to the field of biomolecule detection. More specifically, the present invention relates to compositions, methods and systems for the detection and manipulation of biomolecules using magnetic particles.
46 Citations
23 Claims
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1. A method of identifying the presence of magnetically labeled cell in a sample comprising the steps of:
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providing a sample containing one or more cells;
contacting the target cell with a magnetic label under conditions which permit the formation of a cell-label complex;
subjecting said sample to an applied magnetic field; and
detecting a characteristic response of said sample to an applied magnetic field. - View Dependent Claims (2, 3)
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4. A method for cell detection comprising the steps of:
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providing a cell, contacting the cell with a magnetic probe under conditions which permit the formation of a cell magnetic probe hybrid. identifying the presence of the cell by detecting the magnetic characteristic of the magnetic probe. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11)
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12. A method of assembly of biomolecule comprising the steps of:
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providing a target biomolecule disposed on a support;
providing a probe biomolecule comprising a magnetic label;
contacting the target biomolecule with the probe biomolecule, wherein the probe biomolecule interacts with the target biomolecule; and
applying a magnetic field to the target biomolecule and the probe biomolecule such that the probe biomolecule is induced to move toward the disposed target biomolecule and bind to the target biomolecule. Repeating these steps to produce chains of biomolecules - View Dependent Claims (13, 14, 15)
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16. A method for assaying molecules in a sample comprising the steps of:
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providing a sample which contains one or more target molecules or molecular complexes;
contacting said target with one or more probes under conditions which permit the formation of a target-probe complex, wherein the probe comprises one or more magnetic labels;
subjecting said target-probe complex to an applied magnetic field; and
determining one or more magnetic characteristics of said target-probe complex wherein said sensing means comprises a giant magnetoresistive ratio sensor and flux concentrator with a flux gap; and
the target-probe magnetic label closes the flux gap - View Dependent Claims (17)
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18. A method for assaying molecules in a sample comprising the steps of:
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providing a sample which contains one or more target molecules or molecular complexes;
contacting said target with one or more probes under conditions which permit the formation of a target-probe complex, wherein the probe comprises one or more magnetic labels;
subjecting said target-probe complex to an applied magnetic field; and
determining one or more magnetic characteristics of said target-probe complex wherein said sensing means comprises a giant magnetoresistive ratio sensor and flux concentrator. Wherein the flux concentrator is a cone shape with the small end attached to the giant magnetoresistive ratio sensor and the larger end in close proximity to the target-probe complex. - View Dependent Claims (19, 20)
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21. A method of enhancing the binding of a probe biomolecule to a target biomolecule comprising the steps of:
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providing a target biomolecule disposed on a support;
providing a probe biomolecule comprising a magnetic label;
contacting the target biomolecule with the probe biomolecule, wherein the probe biomolecule interacts with the target biomolecule; and
applying a magnetic field to the target biomolecule and the probe biomolecule such that the probe biomolecule is induced to move toward the disposed target biomolecule. applying a vibration static or changing frequency to the target biomolecule and the probe biomolecule such that the probe biomolecule is induced to move toward the disposed target biomolecule. - View Dependent Claims (22, 23)
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Specification