Method of binding a compound to a sensor surface

• US 20040224137A1
• Filed: 01/23/2004
• Published: 11/11/2004
• Est. Priority Date: 07/23/2001
• Status: Active Grant

• Current Assignee: Applied Nanosystems B.V.
• Original Assignee: Applied Nanosystems B.V.
• Inventors: Rink, Rick, Scholtmeijer, Karin, Wemer, Johannes, Hektor, Harm Jan, Tagu, Denis Etienne Marie Andre, Rogalska, Ewa Maria, Walcarius, Alain Georges Ghislain, Bilewicz, Renata
• Granted Patent: US 7,393,448 B2
• Time in Patent Office: Days
• US Class Current: 428/209
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 428/24917 including metal layer
• CPC Class Codes: Y10T 428/24926 including ceramic, glass, porcelain or quartz layer
• CPC Class Codes: Y10T 428/31504 Composite [nonstructural laminate]
• CPC Class Codes: Y10T 428/31678 Of metal

Abstract

The present invention relates to a method of non-covalently binding a compound to a sensor surface. The method comprises adsorbing a hydrophobin-like substance to at least a part of the sensor surface, such as an electrode. The invention further provides a sensor having a surface, at least a part of which is provided with a coating of a hydrophobin-like substance that comprises a non-covalently bonded compound.

Claims

1. A method of associating a compound with a sensor surface, said method comprising:

coating at least a part of a surface of a sensor with a hydrophobin-like substance; and

contacting the hydrophobin-like substance with the compound to form a non-covalent bond between the hydrophobin-like substance and the compound.

2. The method according to claim 1, wherein the compound is smaller than about 15000 daltons.

3. The method according to claim 1, wherein the compound is smaller than about 2000 daltons.

4. The method according to claim 2, wherein the compound is incorporated in the hydrophobin-like substance.

5. The method according to claim 1, wherein the compound is an electroactive compound.

6. The method according to claim 1, wherein the compound is capable of being converted into an electroactive compound.

7. The method according to claim 1, wherein the compound is larger than about 15000 daltons.

8. The method according to claim 1, wherein the compound comprises a proteinaceous substance.

9. The method according to claim 7, wherein the compound comprises an enzyme.

10. The method according to claim 7, wherein the compound comprises an antibody.

11. The method according to claim 7, wherein the compound comprises a receptor.

12. The method according to claim 1, wherein the compound is at least partly hydrophobic or comprises a hydrophobic anchor.

13. The method according to claim 1, further comprising covalently binding a second compound to the hydrophobin-like substance.

14. The method according to claim 1, further comprising:

non-covalently binding a second compound to the hydrophobin-like substance through a third compound;

wherein the third compound comprises an intermediate compound having an affinity for the second compound.

15. A sensor, comprising:

a surface; and

a coating comprising a hydrophobin-like substance and a compound non-covalently bound to the hydrophobin-like substance;

wherein the coating covers at least part of the surface.

16. The sensor of claim 15, wherein the compound is smaller than about 15000 daltons.

17. The sensor of claim 15, wherein the compound is larger than about 15000 daltons.

18. The sensor of claim 15, wherein the compound is a proteinaceous substance.

19. The sensor of claim 15, further comprising an electrode.

20. The sensor of claim 19, wherein the electrode is selected from the group consisting of a glassy-carbon electrode, a glass electrode and a Thin Mercury Film Electrode.

21. The sensor of claim 15, further comprising an electroactive compound.

22. A method for determining a voltage difference in a solution, the method comprising:

contacting the solution with a sensor comprising;

a surface;

a coating comprising a hydrophobin-like substance and a compound non-covalently bound to the hydrophobin-like substance;

wherein the coating covers at least part of the surface;

a sensor; and

recording a current.