Systems and methods for characterizing a biological condition or agent using selected gene expression profiles
First Claim
1. A method, for evaluating a biological condition affected by a first agent based on a first sample obtained from a target population of cells to which the first agent has been administered, the first sample providing a source of RNAs, the method comprising:
- deriving from the first sample a first profile data set, the first profile data set including a plurality of members, each member being a quantitative measure of the amount of a distinct RNA or protein constituent in a panel of constituents selected so that measurement of the constituents enables evaluation of the biological condition affected by the first agent, wherein such measure for each constituent is obtained under measurement conditions that are substantially similar; and
producing a calibrated profile data set for the panel, wherein each member of the calibrated profile data set is a function of a corresponding member of the first profile data set and a corresponding member of a first baseline profile data set for the panel, and wherein the first baseline profile is related to the biological condition to be evaluated affected by the first agent, the calibrated profile data set being a comparison between the first profile data set and the baseline profile data set, thereby providing an evaluation of the biological condition affected by the agent.
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Abstract
Methods are provided for evaluating a biological condition of a subject using a calibrated profile data set derived from a data set having a plurality of members, each member being a quantitative measure of the amount of a subject'"'"'s RNA or protein as distinct constituents in a panel of constituents. The biological condition may be a naturally occurring physiological state or may be responsive to treatment of the subject with one or more agents. Calibrated profile data sets may be used as a descriptive record for an agent.
35 Citations
86 Claims
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1. A method, for evaluating a biological condition affected by a first agent based on a first sample obtained from a target population of cells to which the first agent has been administered, the first sample providing a source of RNAs, the method comprising:
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deriving from the first sample a first profile data set, the first profile data set including a plurality of members, each member being a quantitative measure of the amount of a distinct RNA or protein constituent in a panel of constituents selected so that measurement of the constituents enables evaluation of the biological condition affected by the first agent, wherein such measure for each constituent is obtained under measurement conditions that are substantially similar; and
producing a calibrated profile data set for the panel, wherein each member of the calibrated profile data set is a function of a corresponding member of the first profile data set and a corresponding member of a first baseline profile data set for the panel, and wherein the first baseline profile is related to the biological condition to be evaluated affected by the first agent, the calibrated profile data set being a comparison between the first profile data set and the baseline profile data set, thereby providing an evaluation of the biological condition affected by the agent. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86)
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Specification