Infectious clones of RNA viruses and vaccines and diagnostic assays derived thereof
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Abstract
An infectious clone based on the genome of a wild-type RNA virus is produced by the process of providing a host cell not susceptible to infection by the wild-type RNA virus, providing a recombinant nucleic acid based on the genome of the wild-type RNA virus, transfecting the host cell with the recombinant nucleic acid and selecting for infectious clones. The recombinant nucleic acid comprises at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either. The infectious clones can be used in single or dual purpose vaccines and in viral vector vaccines.
125 Citations
26 Claims
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1-20. -20. (canceled).
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21. An isolated nucleic acid comprising a DNA sequence encoding an infectious RNA molecule encoding a North American PRRS virus, wherein said DNA sequence is SEQ ID NO:
- 24 or a sequence that hybridizes to the complement of SEQ ID NO;
24 under conditions comprising hybridization to filter-bound DNA in 0.5 M NaHPO4, 7% SDS, 1 mM EDTA at 65°
C., and washing in 0.1×
SSC/0.1% SDS at 68°
C.
- 24 or a sequence that hybridizes to the complement of SEQ ID NO;
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22. A transfected host cell comprising a DNA sequence encoding an infectious RNA molecule encoding a North American PRRS virus, wherein said DNA sequence is SEQ ID NO:
- 24 or a sequence that hybridizes to the complement of SEQ ID NO;
24 under conditions comprising hybridization to filter-bound DNA in 0.5 M NaHPO4, 7% SDS, 1 mM EDTA at 65°
C., and washing in 0.1×
SSC/0.1% SDS at 68°
C., which transfected host cell is capable of expressing the encoded North American PRRS virus.
- 24 or a sequence that hybridizes to the complement of SEQ ID NO;
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23. An isolated nucleic acid comprising a DNA sequence encoding an infectious RNA molecule encoding a North American PRRS virus, wherein said DNA sequence is SEQ ID NO:
- 24.
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24. An isolated nucleic acid in the form of a plasmid, wherein said isolated nucleic acid comprises a DNA sequence encoding an infectious RNA molecule encoding a North American PRRS virus, wherein said DNA sequence is SEQ ID NO:
- 24.
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25. An isolated infectious RNA molecule encoded by an isolated nucleic acid comprising SEQ ID NO:
- 24, which infectious RNA molecule encodes a North American PRRS virus.
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26. A recombinant North American PRRS virus encoded by an isolated nucleic acid comprising a DNA sequence encoding an infectious RNA molecule encoding a North American PRRS virus, wherein said DNA sequence is SEQ ID NO:
- 24.
Specification
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Application NumberUS10/750,409Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current435/6CPC Class CodesA61K 39/00 Medicinal preparations cont...C07K 14/005 from virusesC12N 2770/10022 New viral proteins or indiv...C12N 2770/10034 Use of virus or viral compo...
