Sensors, and methods of making and using the same
First Claim
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1. A compound comprising a first fluorophore, a second fluorophore and a cleavable linker covalently linking the first fluorophore and the second fluorophore, wherein the first fluorophore is represented by the following generalized structure:
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Abstract
The present invention is directed, in part, to sensors for detecting metal ions, and methods of making and using the same.
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32 Claims
- 1. A compound comprising a first fluorophore, a second fluorophore and a cleavable linker covalently linking the first fluorophore and the second fluorophore, wherein the first fluorophore is represented by the following generalized structure:
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12. A compound comprising (a) a first fluorophore comprising a first Lewis base capable of forming a coordination bond with a metal ion, wherein one or more fluorescence properties of the first fluorophore changes upon coordination of the first Lewis base to a metal ion;
- (b) a second fluorophore; and
(c) a cleavable linker covalently linking the first fluorophore and the second fluorophore, wherein the cleavable linker is intended to be cleaved during use of the compound. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
- (b) a second fluorophore; and
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26. A compound represented by the following generalized structure:
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27. A method of detecting, and optionally quantifying the concentration of, a metal ion in a sample or a patient, comprising:
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(a) adding to a sample or administering to a patient one of the compounds claimed above;
(b) measuring fluorescence of the first fluorophore of the compound in the sample or the patient after cleavage of the cleavable linker has occurred for at least some measurable portion of the compound added to the sample or administered to the patient; and
(c) determining whether the metal ion is present in the sample or patient, and optionally the concentration of the metal ion in the sample or patient. - View Dependent Claims (28, 29, 30, 31)
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32. A diagnostic kit for a metal ion, comprising:
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(a) one of the compounds claimed above; and
(b) instructions for using the compound to detect a metal ion in a sample or a patient.
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Specification