Systems and methods for characterizing a biological condition or agent using selected gene expression profiles

• US 20040225449A1
• Filed: 02/17/2004
• Published: 11/11/2004
• Est. Priority Date: 06/28/1999
• Status: Abandoned Application

• Current Assignee: Source Precision Medicine Inc.
• Original Assignee: Source Precision Medicine Inc.
• Inventors: Bankaitis-Davis, Danute M., Cheronis, John C., Tryon, Victor, Bevilacqua, Michael P.
• Application Number:
  US10/781,558
• Publication Number:
  US 20040225449A1
• Time in Patent Office: Days
• US Class Current: 702/20
• CPC Class Codes: G01N 33/5088 of vertebrates
• CPC Class Codes: G01N 33/6803 General methods of protein analysis not limited to specific proteins or families of proteins

Abstract

Methods are provided for evaluating a biological condition of a subject using a calibrated profile data set derived from a data set having a plurality of members, each member being a quantitative measure of the amount of a subject'"'"'s RNA or protein as distinct constituents in a panel of constituents. The biological condition may be a naturally occurring physiological state or may be responsive to treatment of the subject with one or more agents. Calibrated profile data sets may be used as a descriptive record for an agent.

Claims

1. A method for determining a profile data set for a subject, based on a sample from the subject, the sample providing a source of RNAs, the method comprising:

quantitatively measuring an amount of RNA corresponding to each of at least two constituents from any one of Tables 1 through 8, where the profile data set comprises the measure of each constituent and wherein such measure for each constituent is obtained under measurement conditions that are substantially reproducible.

2. A method according to claim 1, wherein the measurement conditions that are reproducible include intra-assay variability and inter-assay variability.

3. A method according to claim 2, wherein intra-assay variability is reproducible such that the average coefficient of variation for repeated measurements for each constituent from the same sample is less than 1 percent.

4. A method according to claim 2, wherein inter-assay variability is reproducible such that the average coefficient of variation for measurements for each constituent in distinct samples of the same material is less than 2 percent.

5. A method according to claim 1, wherein the profile data set is informative about a biological condition for the subject with respect to the circumstances of the subject at the time the sample was obtained.

6. A method according to claim 5, wherein the profile data set provides guidance for medical or surgical intervention by allopathic or naturpathic means.