Magnetic assisted detection of magnetic beads using optical disc drives

• US 20040226348A1
• Filed: 07/24/2002
• Published: 11/18/2004
• Est. Priority Date: 07/24/2001
• Status: Abandoned Application

• Current Assignee: Nagaoka & Co., Ltd.
• Original Assignee: Nagaoka & Co., Ltd.
• Inventors: Norton, James Rodney, Sasaki, Glenn, Bruce, Phillip III, Worthington, Mark Oscar
• Application Number:
  US10/205,005
• Publication Number:
  US 20040226348A1
• Time in Patent Office: Days
• US Class Current: 73/53.01
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

The present invention is a method and apparatus for precise control of magnetic beads in an optical bio-disc. Embodiments of the present invention use electromagnets for precise control of the forces experienced by the magnetic beads. The use of electromagnets eliminates the need to design precise flow control mechanisms to keep beads in place. This is critical in the stage of washing in an assay, where beads attached to a bottom testing surface are separated from beads that are unattached. One embodiment contains a top electromagnet in a top layer of a bio-disc and a bottom electromagnet in a bottom layer of the bio-disc. Another embodiment is an apparatus of electromagnets that can be used to control the magnetic beads within the optical bio-disc. By adjusting the current flow to the electromagnets, precise control of beads can be accomplished.

Claims

1. A method of controlling magnetic beads, said method comprising the steps of:

obtaining an optical disc with at least one detection area;

embedding a top electromagnet in a cap layer of said disc over said detection area; and

embedding a bottom electromagnet in a bottom substrate of said disc under said detection area.

2. The method of claim 1 further comprising the steps of:

placing assay solution into said detection area;

placing magnetic beads into said detection area;

turning on said bottom electromagnet to pull said magnetic beads to the bottom surface of said detection area;

allowing a plurality of said magnetic beads to attach to said bottom surface;

turning off said bottom electromagnet;

turning on said top electromagnet to a specified power whereby unattached beads are pulled to the top surface of said detection area and attached beads remain attached to said bottom surface of said detection area.

3. The method of claim 2 wherein said specified power is less than the non-covalent bonds between said bottom surface and said attached beads.

4. The method of claim 2 wherein said step of allowing further comprises the step of washing said assay solution from said detection area with a wash solution.

5. The method of claim 1 wherein said top electromagnet is attached to a battery source with power control.

6. The method of claim 1 wherein said bottom electromagnet is attached to a battery source with power control.

7. The method of claim 1 wherein said top electromagnet is embedded in a top arm in a holding apparatus over said detection area said optical disc.

8. The method of claim 7 wherein said top electromagnet is attached to a battery source with power control.

9. The method of claim 1 wherein said bottom electromagnet is embedded in a bottom arm in a holding apparatus under said detection area said optical disc.

10. The method of claim 9 wherein said bottom electromagnet is attached to a battery source with power control.

11. An optical bio-disc for performing assays, comprising;

a cap layer;

a middle layer comprising at least one fluidic channel;

a bottom substrate;

a top electromagnet embedded in said cap layer positioned over said fluidic channel; and

a bottom electromagnet embedded in said bottom substrate positioned over said fluidic channel.

12. The optical bio-disc of claim 11 wherein said top electromagnet is attached to a battery source with power control embedded in said cap layer.

13. The optical bio-disc of claim 11 wherein said bottom electromagnet is attached to a battery source with power control embedded in said bottom substrate.

14. A holding apparatus comprising;

a top arm;

a bottom arm;

a stand connecting said top and bottom arms;

a top electromagnet embedded in said top arm;

a bottom electromagnet embedded in said bottom arm;

a surface on said bottom arm facing said top arm whereby an optical bio-disc with assay solution and magnetic beads is placed; and

a battery source with power control for directing current into said top and bottom electromagnets for controlling the movement of said magnetic beads.