Multi-dimensional electrophoresis apparatus
First Claim
1. An electrophoresis apparatus comprising:
- an inlet opening for introducing a sample to be analyzed;
a transport capillary extending in a longitudinal direction;
at least one separation capillary disposed at a pre-selected angle relative to said transport capillary;
at least one analyte concentrator positioned adjacent to said transport capillary and said separation capillary;
said analyte concentrator including a plurality of microstructures;
said microstructures carrying at least one affinity element adapted to attract at least one analyte of interest from said sample which passes through said analyte concentrator; and
a detector means for identifying and characterizing said analyte of interest.
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Accused Products
Abstract
An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes.
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Citations
28 Claims
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1. An electrophoresis apparatus comprising:
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an inlet opening for introducing a sample to be analyzed;
a transport capillary extending in a longitudinal direction;
at least one separation capillary disposed at a pre-selected angle relative to said transport capillary;
at least one analyte concentrator positioned adjacent to said transport capillary and said separation capillary;
said analyte concentrator including a plurality of microstructures;
said microstructures carrying at least one affinity element adapted to attract at least one analyte of interest from said sample which passes through said analyte concentrator; and
a detector means for identifying and characterizing said analyte of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An electrophoresis apparatus comprising:
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an inlet opening for introducing a sample to be analyzed;
a transport channel extending in a longitudinal direction;
at least one separation channel disposed at a pre-selected angle relative to said transport channel;
said transport channel and said separation channel being formed in a microchip;
an analyte concentrator positioned adjacent to said transport channel and said separation channel;
said analyte concentrator including a plurality of microstructures;
said microstructures carrying at least one affinity element adapted to attract at least one analyte of interest from said sample which passes through said analyte concentrator; and
a detector means for identifying and characterizing said analytes of interest. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification