Expression profiling
First Claim
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1. A method for determining a developmental or physiological stage of an organism comprising determining the expression of at least a first gene and a second gene, or gene fragment, said method comprising:
- providing at least a first nucleic acid template derived from said first gene and a second nucleic acid template derived from said second gene hybridizing at least one first primer to said first template and at least one second primer to said second template; and
determining binding of said primers to said templates in one reaction vessel.
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Abstract
The invention relates to DNA sequencing methods useful in expression profiling. The invention provides a semi-automated high-throughput testing facility for expression profiling with immediate use in expression profiling such as in testing plant quality of horticultural and agricultural products.
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Citations
17 Claims
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1. A method for determining a developmental or physiological stage of an organism comprising determining the expression of at least a first gene and a second gene, or gene fragment, said method comprising:
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providing at least a first nucleic acid template derived from said first gene and a second nucleic acid template derived from said second gene hybridizing at least one first primer to said first template and at least one second primer to said second template; and
determining binding of said primers to said templates in one reaction vessel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification