High efficiency transfection based on low electric field strength, long pulse length
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Abstract
A method is provided for introducing nucleic acid into a cell, by contacting the cell with a nucleic acid and applying a low electrical field impulse for a long pulse length. A method is provided for introducing a polypeptide into a cell, by contacting the cell with the polypeptide and applying a low electrical field impulse for a long pulse length.
32 Citations
43 Claims
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1-29. -29. (cancelled)
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30. A method for introducing nucleic acid molecules into cells of a mammalian subject in vivo comprising:
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a. introducing a composition comprising isolated nucleic acid molecules into a mammalian subject;
b. selecting electroporation conditions comprising an electric field having strength of about 300 V/cm to about 600 V/cm delivered in one or more electrical pulses having a pulse length of about 10 milliseconds to about 100 milliseconds; and
c. electroporating tissue comprising cells into which the nucleic acid molecules are to be introduced using the selected electroporation conditions, thereby allowing the nucleic acid molecules to be introduced into the cells. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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Specification