System

• US 20040234940A1
• Filed: 07/19/2004
• Published: 11/25/2004
• Est. Priority Date: 06/18/2001
• Status: Abandoned Application

• Current Assignee: Pig Improvement Company UK Ltd. (Genus PLC)
• Original Assignee: Pig Improvement Company UK Ltd. (Genus PLC)
• Inventors: Van Der Steen, Hein, Anderson, Jon E., Abeydeera, Lalantha R.
• Application Number:
  US10/481,249
• Publication Number:
  US 20040234940A1
• Time in Patent Office: Days
• US Class Current: 435/2
• CPC Class Codes: C12M 21/06 for in vitro fertilization
• CPC Class Codes: C12M 23/16 Microfluidic devices; Capillary tubes integrated microfluidic structures B01L3/5027; microreactors B01J19/0093
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis

Abstract

A micro chamber arrangement for development of oocytes, fertilisation and embryo culture. Objects are kept in a micro environment. The system monitors and regulates the environment for the objects and incorporates regular quality control. The device is very suitable for large scale embryo production. The arrangement can be used for follicle sorting, follicular growth, oocyte maturation, oocyte enucleation, in vitro fertilisation, cloning, nuclear transfer, genetic modification, embryo culture, embryo encapsulation and embryo transport.

Claims

1. Apparatus for handling and/or treatment of cells, in particular follicles, oocytes and/or embryos comprising of at least one

microchamber arrangement containing a plurality of microchambers, each optionally comprising one or more sieve elements, wherein the micro chamber arrangement further incorporates a pump that controls the recirculation of medium and optionally devices to measure and/or regulate characteristics of the medium, and/or introduce spermatozoa.

2. Apparatus as claimed in claim 1 further comprising a chamber containing a plurality of sieve elements arranged in succession within the chamber, wherein each successive sieve element has pores of a smaller dimension than those in the preceding sieve element, connected to a pump to maintain a circulatory flow of medium, wherein said sieve elements separate primordial follicles, preantral follicles or Cumulus-Oocyte-Complexes (COCs) from ovarian debris and sorts the follicles according to size.

3. Apparatus as claimed in claim 1 or claim 2 wherein the characteristics are any one or more of pH, osmolarity, carbon dioxide levels, and temperature.

4. Apparatus as claimed in claim 1 wherein the microchamber sieve elements are adjustable, such that the pore size can be altered, or the pores can be closed.

5. Apparatus as claimed in claim 1 wherein the microchamber sieve elements have pores of different dimension.

6. Apparatus as claimed in claim 1 wherein the microchamber arrangement comprises a series of layers of microchambers.

7. Apparatus as claimed in claim 6 wherein sieve elements associated with each layer of microchambers form a plurality of sieve elements arranged in succession, wherein each successive sieve element has pores of a larger pore dimension than those in the preceding sieve element.

8. Apparatus as claimed in claim 6 wherein sieve elements associated with each layer of microchambers form a plurality of sieve elements arranged in succession, wherein each successive sieve element has pores of a smaller pore dimension than those in the preceding sieve element.

9. Apparatus as claimed in claim 6 successive layers contain microchambers of decreasing dimension.

10. Apparatus as claimed in claim 1, wherein the connecting walls of said microchambers contain holes to allow the flow of medium in and/or out of each individual microchamber from the sides.

11. Apparatus as claimed in claims 1 wherein the microchamber arrangement is linked to an imaging means linked to an image capturing device.

12. Apparatus as claimed in claim 10 wherein the imaging means is a video camera and the image capturing device is a microscope or uses ultra sound to generate a quality index based on visual assessment and other parameters such as medium pH, medium osmolarity, medium temperature, and/or oocyte/embryo metabolism.

13. Apparatus as claimed in claim 11 wherein the imaging is linked to other devices that monitor characteristics such as medium pH, temperature, osmolarity and/or oocyte/embryo metabolism to generate a quality index for each said oocyte/embryo.

14. Apparatus as claimed in claim 4 wherein the microchamber arrangement is positioned above a transfer device.

15. Apparatus as claimed in claim 13 wherein the transfer device is a second microchamber arrangement or straw.

16. Apparatus as claimed in claim 1 wherein the micro chamber arrangement further incorporates a second pump system.

17. Apparatus as claimed in claim 15 wherein the second pump regulates the introduction of a small number of spermatozoa into the micro chambers.

18. Apparatus as claimed in claim 6 wherein the microchamber arrangement further incorporates oocyte/embryo encapsulation means.

19. Apparatus as claimed in claim 1 wherein each microchamber consists of two sub-chambers.

20. Apparatus as claimed in claim 18 wherein the subchamber is formed to have dimensions equivalent to or greater than a mature oocyte.

21. The use of the apparatus as claimed in claim 1 in one or more of:

(a) Separation of follicles from ovaries

(b) Growth of follicles

(c) Removal of COCs from follicles

(d) Maturation of COCs (IVM)

(e) Removal of cumulus cells from COC'"'"'s

(f) Oocyte enucleation

(g) In vitro fertilisation

(h) Nuclear transfer (NT)/cloning

(i) Embryo culture

(j) Splitting embryos

(k) Sorting embryos and or oocytes according to size

(l) Encapsulation

(m) Transport of embryos from the production facility to the site of embryo transplantation

(n) Cell culture.

22. The use as claimed in claim 20 when the oocytes or embryos or sperm are from mammals or aquatic species.