Surfaces that selectively bind to moieties
First Claim
1. A thin film having a maximum thickness of 2 microns and comprising a polymer having a flexural modulus of at least 150,000 psi, wherein a surface of the film comprises a plurality of indentations, each indentation comprising a reverse impression of a portion of an outer surface of an imprint moiety, and wherein when the thin film is mounted on a quartz crystal microbalance (QCM), the QCM can detect the imprint moiety in a liquid sample at a minimum concentration of at least as low as 1000 imprint moieties per milliliter of sample.
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Accused Products
Abstract
A thin film made of a stiff polymer and having indentations on its surface, and methods of making and using the thin film are disclosed. The surface includes a plurality of indentations, the shape of each indentation corresponding to an outer surface of an imprint moiety, such as an animal, bacterial, or plant cell. The thin film can be mounted on a detector, which can be used to selectively bind to and detect the imprint moiety in a sample, e.g., a biological or environmental sample, at a minimum concentration of as low as 500 to 1000 imprint moieties per milliliter of sample.
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Citations
32 Claims
- 1. A thin film having a maximum thickness of 2 microns and comprising a polymer having a flexural modulus of at least 150,000 psi, wherein a surface of the film comprises a plurality of indentations, each indentation comprising a reverse impression of a portion of an outer surface of an imprint moiety, and wherein when the thin film is mounted on a quartz crystal microbalance (QCM), the QCM can detect the imprint moiety in a liquid sample at a minimum concentration of at least as low as 1000 imprint moieties per milliliter of sample.
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30. A method of making a thin film, the method comprising
polymerizing one or more monomers in the presence of a plurality of imprint moieties to form a polymer having a flex modulus of at least 150,000 psi; -
forming a sheet of the polymer having a maximum thickness of 2 microns; and
removing the imprint moieties from a surface of the polymer sheet leaving a plurality of indentations on the surface, each indentation comprising a reverse impression of a portion of an outer surface of an imprint moiety;
wherein when the thin film is mounted on a quartz crystal microbalance (QCM), the QCM can detect the imprint moiety in a liquid sample at a minimum concentration of at least as low as 1000 imprint moieties per milliliter of sample. - View Dependent Claims (31, 32)
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Specification