Polymerase chain reaction device and method of regulating opening and closing of inlet and outlet of the polymerase chain reaction device
First Claim
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1. A PCR (polymerase chain reaction) device comprising:
- an inlet through which a biochemical fluid is injected;
an outlet through which the biochemical fluid is discharged;
a PCR channel positioned between the inlet and the outlet;
first and second micro-valves, which control opening and closing of the inlet and the outlet; and
a sol-gel transformable material, which transforms from a sol state into a gel state at a temperature lower than DNA denaturation temperature, annealing temperature and extension temperature and higher than room temperature.
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Abstract
A polymerase chain reaction (PCR) device with micro-valves, which are opened or closed using a simple control mechanism is provided. The PCR device includes: an inlet through which a biochemical fluid is injected; an outlet through which the biochemical fluid is discharged; a PCR channel positioned between the inlet and the outlet; first and second micro-valves, which control opening and closing of the inlet and the outlet; and a sol-gel transformable material, which transforms from a sol state into a gel state at a temperature lower than DNA denaturation temperature, annealing temperature and extension temperature and higher than room temperature.
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Citations
16 Claims
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1. A PCR (polymerase chain reaction) device comprising:
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an inlet through which a biochemical fluid is injected;
an outlet through which the biochemical fluid is discharged;
a PCR channel positioned between the inlet and the outlet;
first and second micro-valves, which control opening and closing of the inlet and the outlet; and
a sol-gel transformable material, which transforms from a sol state into a gel state at a temperature lower than DNA denaturation temperature, annealing temperature and extension temperature and higher than room temperature. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 12, 13, 14, 15, 16)
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10. A method of regulating opening and closing of an inlet and an outlet of a PCR device, the method comprising:
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connecting micro-valves, each of which contains a sol-gel transformable material that transforms from a sol state to a gel state at a temperature lower than DNA denaturation temperature, annealing temperature and extension temperature regarding PCR and higher than room temperature, to the inlet and the outlet of the PCR device; and
inducing a sol-to-gel transformation in the micro-valves using temperature variations in a thermal cycle of PCR. - View Dependent Claims (11)
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Specification