Enrichment methods for the detection of pathogens and other microbes
First Claim
1. A method for remote detection of a pathogen or other microbe in a solid sample, semi-solid sample or liquid sample comprising:
- obtaining a test sample at a first location, the sample being solid, semi-solid or liquid;
diluting, at the first location, the sample with enrichment medium at a ratio between about 1;
0.1 to about 1;
2 (wt./vol.), or greater;
incubating the diluted sample at an optimal temperature in an incubator during transit of the diluted sample to a second location that is a remote test location; and
determining, at the remote test location, by assaying the diluted incubated test sample, or a portion thereof, with an assay suitable to detect the pathogen or other microbe, whether the sample is contaminated.
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Accused Products
Abstract
The present invention provides novel enrichment, testing and detection methods for detection of pathogens or other microbes in a food, water, wastewater, industrial, pharmaceutical, botanical, environmental samples and other types of samples analyzed by enrichment-detection methods. In preferred aspects, a sample is obtained at a first location and is diluted (e.g., in the case of a solid or semi-solid sample. or liquid) at the first location at a ratio of about 1:0 (wt./vol.) to 1:2 (wt./vol.), or greater, preferably at a ratio of about 1:0.1 (wt./vol.) or greater, or more preferably, at a ratio of about 1:2 (wt./vol.) or greater. The diluted sample is incubated at an optimal temperature in an incubator and either tested locally, or sent in a shipping incubator to a second location that is a remote test location. The incubated sample is received and tested at the second location by assaying the sample, or a portion thereof, with an assay suitable to detect the pathogen or other microbe. In alternate embodiments, no dilution at the first location is required, and optionally minimal additions to adjust intrinsic deficiencies may be made, but the sample is nonetheless incubated during transit to the test location.
32 Citations
28 Claims
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1. A method for remote detection of a pathogen or other microbe in a solid sample, semi-solid sample or liquid sample comprising:
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obtaining a test sample at a first location, the sample being solid, semi-solid or liquid;
diluting, at the first location, the sample with enrichment medium at a ratio between about 1;
0.1 to about 1;
2 (wt./vol.), or greater;
incubating the diluted sample at an optimal temperature in an incubator during transit of the diluted sample to a second location that is a remote test location; and
determining, at the remote test location, by assaying the diluted incubated test sample, or a portion thereof, with an assay suitable to detect the pathogen or other microbe, whether the sample is contaminated. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 22, 24)
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14. A method for remote detection of a pathogen or other microbe in a liquid sample comprising:
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obtaining a liquid sample at a first location;
incubating the sample at an optimal temperature in an incubator during transit of the diluted sample to a second location that is a remote test location; and
determining, at the remote test location, by assaying the diluted incubated test sample, or a portion thereof, with an assay suitable to detect the pathogen or other microbe, whether the sample is contaminated. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 23, 26)
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27. A method for remote detection of a pathogen or other microbe in a solid sample, semi-solid sample or liquid sample comprising:
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obtaining a test sample at a first location, the sample being solid, semi-solid or liquid;
diluting, at the first location, the sample with enrichment medium at a range of ratio of about 1;
0.1 to 1;
2 (wt./vol.), or not diluting the sample;
incubating the enriched sample at an optimal temperature in an incubator for testing in a second location that is an in-house or local lab; and
determining, at the in-house or local lab, by assaying the diluted incubated test sample, or a portion thereof, with an assay suitable to detect the pathogen or other microbe, whether the sample is contaminated. - View Dependent Claims (28)
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Specification