Amperometric biosensor in thick film technology
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Abstract
Described is an amperometric biosensor in thick film technology for the detection an/or determination of substances undergoing enzyme catalyzed reactions, especially glucose or lactate, comprising an inert carrier material, at least one transducer layer exhibiting a specific electrical conductivity χ>104 Ω−1 cm−1, and at least one bioactive layer with diffusion barrier function containing at least one enzyme specifically reacting with said substance to be measured, said bioactive layer exhibiting a specific electrical conductivity χ<1 Ω−1 cm−1 and said bioactive layer further effectively hinders other electroactive substances to reach the transducer layer within the time of measurement.
158 Citations
14 Claims
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1-5. -5. (Canceled).
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6. A thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:
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an inert carrier material;
at least one transducer layer exhibiting a specific electrical conductivity χ
>
104 Ω
−
1cm−
1, during use of the biosensor; and
at least one bioactive layer adapted to function as a diffusion barrier during use of the biosensor, the bioactive layer further defined as;
comprising at least one enzyme capable of specifically reacting with the substance to be detected or determined during use of the biosensor;
exhibiting a specific electrical conductivity of χ
<
1 Ω
−
1cm−
1 during use of the biosensor; and
capable of effectively hindering electroactive substances, other than any produced by the substance undergoing the enzyme-catalyzed reaction, from reaching the transducer layer during use of the biosensor. - View Dependent Claims (7, 8, 9)
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10. A process for producing a thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:
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providing an inert carrier material;
depositing at least one electrically conducting layer on the inert carrier material; and
depositing at least one bioactive layer capable of functioning as a diffusion barrier during use of the biosensor onto the at least one electrically conducting layer by screen printing using a transducer-free printing ink comprising an enzyme and a biocompatible polymer, this depositing being accomplished without the use of UV-radiation. - View Dependent Claims (11)
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12. A printing ink comprising an enzyme, wherein the ink is screen printable without the use of UV-radiation and capable of, in an amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction, forming at least one bioactive layer, adapted to:
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function as a diffusion barrier during use of the biosensor;
have a specific electrical conductivity of χ
<
1 Ω
−
1cm−
1 during use of the biosensor; and
effectively hinder electroactive substances other than any produced by the substance undergoing the enzyme-catalyzed reaction from reaching a transducer layer during use of the biosensor. - View Dependent Claims (13)
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14. A method of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:
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obtaining a thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising;
(a) an inert carrier material;
(b) at least one transducer layer exhibiting a specific electrical conductivity χ
>
104 Ω
−
1cm−
1, during use of the biosensor; and
(c) at least one bioactive layer adapted to function as a diffusion barrier during use during use of the biosensor, the bioactive layer further defined as;
(i) comprising at least one enzyme capable of specifically reacting with the substance to be detected or determined during use of the biosensor;
(ii) exhibiting a specific electrical conductivity of χ
<
1 Ω
−
1cm−
1 during use of the biosensor; and
(iii) capable of effectively hindering electroactive substances, other than any produced by the substance undergoing the enzyme-catalyzed reaction, from reaching the transducer layer during use of the biosensor;
obtaining a sample; and
using the biosensor to detect and/or determine a substance in the sample.
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Specification