×

High throughput screening of libraries

  • US 20040241759A1
  • Filed: 10/17/2003
  • Published: 12/02/2004
  • Est. Priority Date: 06/16/1997
  • Status: Abandoned Application
First Claim
Patent Images

1. A method for screening for a ligand binding protein of interest, comprising, a) encapsulating one or more members of a population of cells suspected of expressing a ligand binding protein of interest in a capsule comprising permeable walls, said walls containing a first capture reagent for said ligand binding protein of interest;

  • b) incubating said encapsulated cells under conditions that allow for expression of said ligand binding protein of interest and capture of said ligand binding protein by said first capture reagent;

    c) contacting said permeable capsule with a ligand specific for said captured ligand binding protein, to form a captured protein-ligand complex, wherein said ligand can be the same as or different from said capture reagent;

    d) contacting said captured protein-ligand complex with a first detection molecule that binds said protein-ligand complex to form a protein-ligand-first detection molecule complex;

    e) contacting said protein-ligand-first detection molecule complex with a second detection molecule that binds said first detection molecule to form a protein-ligand-first detection molecule-second detection molecule complex;

    f) contacting said protein-ligand-first detection molecule-second detection molecule complex with a third detection molecule comprising a detectable label that binds to said protein-ligand-first detection molecule-second detection molecule complex to form a protein-ligand-first detection molecule-second detection molecule-third detection molecule complex; and

    g) detecting the presence of said detectable label bound to said capsule, thereby identifying cells in said capsule expressing said ligand binding protein of interest.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×