Method and apparatus for sample preparation using solid phase microextraction

• US 20040241874A1
• Filed: 02/27/2004
• Published: 12/02/2004
• Est. Priority Date: 08/31/2001
• Status: Abandoned Application

• Current Assignee: SGE Analytical Science Pty Ltd.
• Original Assignee: SGE Analytical Science Pty Ltd.
• Inventors: Abdel-Rehim, Mohamed
• Application Number:
  US10/487,984
• Publication Number:
  US 20040241874A1
• Time in Patent Office: Days
• US Class Current: 436/177
• CPC Class Codes: B01L 2200/0631 Purification arrangements, e.g. solid phase extraction [SPE]
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 2030/009 Extraction
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

The present invention relates to a method and apparatus for use in performing sample preparation of a liquid sample using solid phase microextraction comprising the steps of drawing a liquid sample comprising analytes into a syringe having a syringe barrel provided with a solid phase material, passing the liquid sample through the solid phase material such that the analytes are adsorbed to the solid phase material; and eluting the analytes with a liquid solvent directly into the injector of an anlysing instrument. The apparatus for performing sample preparation of a liquid sample using solid phase microextraction comprises a syringe having a syringe barrel with a plunger slidable within the barrel and a hollow needle extending from the barrel. The liquid sample is drawn through the needle into the barrel wherein a solid phase material is provided in the syringe barrel to be contacted by the liquid sample drawn into the syringe.

Claims

1. A method for preparing a liquid sample using solid phase microextraction, the method comprising the steps of:

a) drawing a liquid sample comprising analytes into a syringe, wherein the syringe comprises a syringe barrel containing a solid phase materials;

b) passing the liquid sample through the solid phase material such that the analytes in the sample are adsorbed to the solid phase material; and

c) eluting the analytes with a liquid solvent directly into an injector port of an analyzing instrument.

2. The method according to claim 1, wherein the sample is drawn into the syringe using an autosampler.

3. The method according to claim 1, wherein the solid phase material is washed prior to the eluting step.

4. The method according to claim 1, wherein the analytes are eluted with an organic solvent.

5. An apparatus for preparing a liquid sample using solid phase microextraction, the apparatus comprising:

a syringe having a syringe barrel and a plunger slidable within the barrels;

a hollow needle extending from the barrel, through which needle the liquid sample is drawn into the syringe barrel; and

a solid phase material located in the syringe barrel.

6. The apparatus according to claim 5, wherein the solid phase material is provided as a porous plug inside the syringe barrel.

7. The apparatus according to claim 5, wherein the solid phase material is a coating on the inside of the syringe barrel wall.

8. The apparatus according to claim 5, wherein the solid phase material is a coating on a filter located inside the syringe barrel.

9. The apparatus according to any one of claims 5 to 8, wherein the solid phase material comprises a solid polymer.

10. The apparatus according to any one of claims 5 to 8, wherein the solid phase material comprises a liquid polymer.