Mouse/human chimeric anti-phencyclidine antibody and uses thereof
First Claim
1. A chimeric mouse/human monoclonal antibody comprising human immunoglobulin constant domains and immunoglobulin variable domains of murine antibody.
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Abstract
The present invention provides a chimeric mouse/human antibody (ch-mAb6B5) for treatment of abuse and toxicity of the arylcyclohexylamines class of drugs (i.e., phencyclidine- or PCP-like drugs). This antibody comprises light and heavy chain PCP binding regions of mouse mAb6B5, coupled to the light and heavy chain constant regions of a human kappa IgG2 or IgG4 isoform. Also provided are the DNA and amino acid sequences of the chimeric light and heavy chain of this antibody. Further provided are data that demonstrate that the new chimeric antibody retains the high affinity and specificity of a previously generated mouse anti-PCP monoclonal antibody (mAb6B5) yet being minimally immunogenic since it has human immunoglobulin constant region. This new medication would allow safe and effective treatment of PCP drug overdose, decrease mortality, and reduce harmful effects due to excessive and prolonged PCP drug use.
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Citations
35 Claims
- 1. A chimeric mouse/human monoclonal antibody comprising human immunoglobulin constant domains and immunoglobulin variable domains of murine antibody.
- 15. An expression vector, wherein said expression vector comprises DNA encoding human immunoglobulin light chain constant domain and immunoglobulin variable domain of murine antibody.
- 19. An expression vector, wherein said expression vector comprises DNA encoding human immunoglobulin heavy chain constant domain and immunoglobulin variable domain of murine antibody.
- 23. A host cell line comprising a chimeric light chain expressing vector, wherein said expression vector comprises DNA encoding human immunoglobulin light chain constant domain and variable domain of murine antibody, and a chimeric heavy chain expressing vector, wherein said expression vector comprises DNA encoding human immunoglobulin heavy chain constant domain and variable domain of murine antibody.
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32. A method of producing the recombinant chimeric mouse/human monoclonal antibody, comprising the steps of:
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amplifying the cDNAs of variable domains of murine monoclonal antibody;
constructing chimeric light and heavy chain expression vectors comprising said amplified cDNAs and DNA encoding constant domains of human immunoglobin;
co-transfecting the said expression vectors into a cell; and
culturing the cell under conditions effective for expression of said recombinant chimeric monoclonal antibody. - View Dependent Claims (33, 34, 35)
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Specification