Water soluble extract from plant of solanum genus and the preparation process thereof, and pharmaceutical composition containing the water soluble extract
First Claim
1. A water-soluble extract from a plant of Solanum geuns, consisting essentially of at least 60%-90% of solamargine and solasonine.
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Abstract
A water soluble extract from a plant of Solanum genus consists essentially of at least 60%-90% of solamargine and solasonine. A process for preparing the water soluble extract from the plant of Solanum genus involves the steps of hydrolysis with an acid, precipitation with a base, and separation treatments using chloroform, alcohol and water as extraction solvents. The water soluble extract prepared from the process can be directly dissolved in pure or neutral pH water to form a yellowish clear and transparent aqueous solution having a water solubility ranging from 2˜20 mg/ml or higher.
The water soluble extract can be used as an active component in a pharmaceutical composition for inhibiting the growth of tumor/cancer cells, in particular liver cancer cells, lung cancer cells and breast cancer cells.
22 Citations
59 Claims
- 1. A water-soluble extract from a plant of Solanum geuns, consisting essentially of at least 60%-90% of solamargine and solasonine.
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31. A process for preparing a water-soluble extract from a plant of Solanum genus, comprising the steps of:
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(a) subjecting a plant material of a plant of Solanum geuns to an extraction treatment using an acidic aqueous solution with a pH value of 3˜
5, such that an aqueous solution is obtained;
(b) adjusting the pH value of the aqueous solution obtained in step (a) to pH 8˜
10 with a base, such that a precipitate is formed;
(c) washing the precipitate formed in step (b) with water, followed by drying, such that a dried product is obtained;
(d) admixing the dried product obtained in step (c) with chloroform, followed by addition of a suitable amount of a 100% alcohol, such that a chloroform-alcohol mixture is formed;
(e) mixing the chloroform-alcohol mixture formed in step (d) with a water/alcohol solution having a predetermined water;
alcohol ratio, such that a mixture containing a chloroform-based layer and a non-chloroform-based layer is obtained;
(f) removing the chloroform-based layer from the mixture obtained in step (e), followed by addition of a suitable amount of water; and
(g) obtaining a supernatant from the resultant mixture of step (f), followed by drying the supernatant, wherein the resultant dried product is able to be directly dissolved in water to form a yellowish clear and transparent aqueous solution. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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Specification