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Proximity-mediated rolling circle amplification

  • US 20040248103A1
  • Filed: 06/04/2003
  • Published: 12/09/2004
  • Est. Priority Date: 06/04/2003
  • Status: Abandoned Application
First Claim
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1. A method comprising:

  • (a) bringing into contact one or more analyte samples and one or more binding guide conjugates, wherein each binding guide conjugate comprises a specific binding molecule and a guide oligonucleotide, wherein each specific binding molecule interacts directly or indirectly with an analyte in the analyte sample, and incubating the analyte samples and the binding guide conjugates under conditions that promote interaction of the specific binding molecules and the analytes, (b) prior to, simultaneous with, or following step (a), bringing into contact the binding guide conjugates and one or more half circle probes, wherein each half circle probe comprises a single-stranded DNA molecule comprising two guide complement portions, wherein each guide complement portion is complementary to one of the guide oligonucleotides, and incubating the binding guide conjugates and the half circle probes under conditions that promote hybridization between the guide oligonucleotides and the half circle probes, (c) following step (b) and prior to, simultaneous with, or following step (a), incubating the binding guide conjugates and half circle probes under conditions that promote ligation of half circle probes, thereby producing one or more amplification target circles.

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