Distribution of solutions across a surface

• US 20040248125A1
• Filed: 07/26/2004
• Published: 12/09/2004
• Est. Priority Date: 08/13/2001
• Status: Abandoned Application

• Current Assignee: Vanderbilt University
• Original Assignee: Vanderbilt University
• Inventors: Schaffer, David K., Fisher, Timothy S., Haselton, Frederick R., McQuain, Mark, Stremler, Mark A
• Application Number:
  US10/486,734
• Publication Number:
  US 20040248125A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01F 25/50 Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle
• CPC Class Codes: B01F 31/65 the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0605 check valves
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: Y10T 137/2544 Supply and exhaust type

Abstract

A system 10 is provided for improved microarray biomolecular analysis. A microarray 36 is placed in a shallow chamber 20, and an induced motion of test fluid through the chamber is achieved by a sequential series of pulses directed to a plurality of source-sink pairs.

Claims

1. A method for distributing a fluid across a surface, comprising:

(a) providing a shallow planar chamber having x and y dimensions, and having a z dimension perpendicular to the x and y dimensions, the z dimension being no greater than {fraction (1/10)} of either of the x or y dimensions;

(b) providing at least one source-sink pair of fluid connections to the chamber, the source and sink of each pair being spaced along the x and/or y dimensions;

(c) providing within the chamber a probe surface having a plurality of probes defined thereon, the probes being spaced across the x and/or y dimensions of the chamber; and

(d) pulsing a test fluid through the chamber in a series of pulses via the at least one source-sink pair and thereby creating a motion of the test fluid across the probe surface.

2. The method of claim 1, wherein:

step (b) comprises providing at least a second source-sink pair of fluid connections to the chamber.

3. The method of claim 2, wherein step (d) further comprises:

(d)(1) injecting test fluid into the chamber through the first source, for a first time interval, and simultaneously extracting test fluid from the first sink; and

(d)(2) after (d)(1), injecting test fluid into the chamber through the second source, for a second time interval, and simultaneously extracting test fluid from the second sink.

4. The method of claim 3, further comprising repeating steps (d)(1) and (d)(2).

5. The method of claim 4, further comprising varying the first and second time intervals.

6. The method of claim 3, wherein:

in step (d)(2), at least part of the test fluid injected into the chamber is test fluid which was extracted from the chamber in step (d)(1).

7. The method of claim 1, wherein the motion of test fluid across the probe surface is laminar flow.

8. The method of claim 1, further comprising:

during step (d), varying at least one boundary of the chamber.

9. The method of claim 1, wherein:

in step (c), the probe surface is a surface of a microarray having an array of biological and/or chemical probe materials immobilized on the microarray surface.

10. The method of claim 9, wherein:

in step (d), the test fluid includes a liquid solution carrying a plurality of particles of test material, and the motion of the test fluid causes a majority of the probes to be contacted by a majority of the particles of test material.

11. A system for distributing a fluid across a surface, comprising:

a test chamber having length and width dimensions at least an order of magnitude greater than a maximum depth dimension;

first and second fluid inlets to the chamber and first and second fluid outlets from the chamber;

a probe surface disposed in the chamber and having a plurality of samples of probe materials located on the probe surface; and

a test fluid flow control assembly connected to the fluid inlets and fluid outlets, so that test fluid may be supplied to the chamber in a sequence of pulses directed to the first and second fluid inlets, the first and second fluid inlets being operably associated with the first and second fluid outlets, respectively, so that when fluid flows in the first fluid inlet fluid simultaneously flows out the first fluid outlet.

12. The system of claim 11, wherein the test fluid flow control assembly further comprises:

a common fluid conduit external of the chamber and connecting the first fluid inlet with the second fluid outlet;

an inlet check valve connected to the first fluid inlet for preventing fluid from flowing out of the first fluid inlet into the common fluid conduit; and

an outlet check valve connected to the second fluid outlet for preventing fluid from flowing from the common fluid conduit into the second fluid outlet.

13. The system of claim 12, further comprising:

a second common fluid conduit external of the chamber and connecting the second fluid inlet with the first fluid outlet;

a second inlet check valve connected to the second fluid inlet; and

a second outlet check valve connected to the first fluid outlet.

14. The system of claim 13, further comprising:

at least one pump connected to the first and second common fluid conduits for sequentially injecting fluid into the first and second inlets.

15. A microarray biomolecular analysis apparatus, comprising:

a chamber for receiving a microarray, the chamber including at least two fluid inlets and at least two fluid outlets; and

a flow control system connected to the fluid inlets and fluid outlets of the chamber for providing test fluid to the chamber in a sequential series of pulses including a first pulse in which fluid enters the first fluid inlet and simultaneously exits the first fluid outlet, and a second pulse in which fluid enters the second fluid inlet and simultaneously exits the second fluid outlet.

16. The apparatus of claim 15, wherein the flow control system further comprises:

check valves associated with each of the fluid inlets and fluid outlets.

17. The apparatus of claim 15, wherein the flow control system further comprises:

at least one pump for alternatingly injecting fluid into the first and second fluid inlets.

18. The apparatus of claim 15, wherein the flow control system further comprises a pulse interval adjustment for varying a length of time during which fluid is injected during each sequential pulse.

19. A method of distributing fluid, comprising:

(a) providing a working fluid volume;

(b) providing in the working fluid volume a probe surface having a plurality of probe samples of biological and/or chemical materials located on the probe surface;

(c) extracting at least a portion of the fluid from the working fluid volume;

(d) reinjecting at least part of the fluid extracted in step (c) back into the working fluid volume;

(e) repeating steps (c) and (d); and

(f) thereby distributing the fluid across the probe surface.

20. The method of claim 19, wherein:

in step (c), fluid is extracted from the working fluid volume at a first point; and

in step (d), fluid is reinjected into the working fluid volume at the first point.

21. The method of claim 19, wherein:

in step (c), fluid is extracted from the working fluid volume at a first point; and

in step (d), fluid is reinjected into the working fluid volume at a second point different from the first point.

22. The method of claim 19, wherein the working fluid volume varies over time.

23. The method of claim 19, wherein the working fluid volume is constant.

24. The method of claim 23, wherein:

simultaneously with step (c), an equal amount of fluid is injected into the working fluid volume.