Releasable polymer arrays
First Claim
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2-1. The method of claim 3 wherein said photogroup is activated by light having a wavelength of 313 m and below.
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Abstract
Methods are provided for fabricating an array of polymers wherein the polymers may be released from the surface of the array by activation of a cleavable moiety. Also provided are arrays of polymers having of polymers wherein the polymers can be released from the surface of the array by activation of a releasable group. Arrays of nucleic acids wherein a nucleic acid probe may be released from the array by activation of a releasable groups and methods for fabrication of such arrays are also disclosed.
16 Citations
31 Claims
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2-1. The method of claim 3 wherein said photogroup is activated by light having a wavelength of 313 m and below.
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3-2. The method of claim 3 wherein said photogroup is activated by light having a wavelength of about 313 nm and below, but not above 313 mm.
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8. A method for releasing polymers from an array of polymers on a solid substrate, said method comprising the steps of:
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providing a solid substrate;
attaching a plurality of linkers to said solid substrate, said solid substrate having a surface, each said linker comprising a cleavable moiety, wherein said cleavable moiety is activatable only at a distinct set of conditions and wherein activation of said cleavable moiety disrupts the linker to allow release of the polymer from the array, to provide a plurality of attached linkers and wherein each said linker has two terminal ends, the first end of which is attached to the substrate and the second end of which is away from the substrate and comprises a reactive group covered by a photoprotective removable group having a first activation energy wavelength;
selectively exposing said photoprotective removable group on said attached linkers to light to selectively remove said photoprotective groups and provide unprotected reactive groups in one or more predefined regions;
exposing under reactive conditions said one or more predefined regions with exposed reactive groups to a first monomer and attaching said first monomer to the exposed reactive groups, wherein sad first monomer comprises a reactive group protected by a photoprotective removable group having said first activation energy wavelength;
selectively exposing said photoprotective removable groups on said attached linkers or said attached first monomer to light to selectively remove said photoprotective groups and expose reactive groups in one or more predefined regions;
exposing under reactive conditions said one or more predefined regions with exposed reactive groups to a second monomer and attaching said second monomer to said exposed groups, wherein sad second monomer comprises a reactive group protected by a photoprotective removable group having a first activation energy wavelength;
repeating said steps of selectively exposing photoprotective removable groups and exposing reactive groups to further monomers each compising a reactive group protected by a photoprotective removable group until the desired array of polymers is complete and subjecting the array to the distinct set of conditions to release the array of polymers. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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- 15. A releasable polymer array comprising a substrate having a linker comprising a cleavable moiety which is labile under a set of conditions and attached to said linker is a polymer, wherein said polymer can be released by exposure of the array to the set of conditions.
- 22. A polymer array having releasable polymers, said array comprising a substrate having attached thereto polymers, wherein one or more of said polymers comprises a cleavable moiety which is labile under a distinct set of conditions wherein said releasable group allows release of the polymer upon activation.
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29. A method for fabricating a polymer array having releasable polymers, said method comprising the steps of:
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providing a substrate;
attaching a plurality of linkers to said substrate, said linkers comprising a cleavable moiety which is labile under a distinct set of conditions;
reversibly modifying said cleavable moiety with a protecting group to provide a reversibly modified cleavable moiety wherein said modified cleavable moiety is not labile under the distinct set of conditions;
attaching a first monomer to said linker;
attaching a second monomer to said linker or to the first monomer;
repeating said step of attaching said further monomer until the desired array of polymers is complete; and
demodifying said reversibly modified releasable group. - View Dependent Claims (30, 31)
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Specification