Novel family of beta sub-unit proteins from a voltage-gated sodium channel, nucleic acids encoding them and therapeutic or diagnostic uses thereof
First Claim
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1. A purified or isolated nucleic acid encoding a β
- 3 sub-unit from a voltage-gated sodium channel, or a sequence complementary thereto.
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Abstract
The present invention relates to a novel family of beta sub-unit proteins from a voltage-gated sodium channel, and particularly the human and the rat beta sub-units which have been collectively identified as β3, in view of their close structural relationship.
The invention also deals with the use of the β3 sub-unit polypeptide or a fragment thereof as well as of the nucleic acids encoding same for therapeutic, diagnostic and screening purposes.
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Citations
38 Claims
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1. A purified or isolated nucleic acid encoding a β
- 3 sub-unit from a voltage-gated sodium channel, or a sequence complementary thereto.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 35)
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14. A purified or isolated polynucleotide comprising at least 10 consecutive nucleotides of a nucleic acid encoding a β
- 3 sub-unit of a voltage-gated sodium channel, with the exception of the polynucleotide bearing SEQ ID NO 876 in W09845435 and the polynucleotide of EMEST database having accession NO AA685538.
- View Dependent Claims (15, 16, 17)
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30. A purified or isolated polypeptide comprising the amino acid sequence of the β
- 3 sub-unit from a voltage-gated sodium channel, or a peptide fragment thereof.
- View Dependent Claims (31, 32)
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33. A purified or isolated polypeptide comprising an amino acid sequence having at least 90% amino acid identity with the amino acid sequence of SEQ ID NO 1 over the entire length of the sequence of SEQ ID NO 1, or a peptide fragment thereof.
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34. A purified or isolated polypeptide comprising an amino acid sequence having at least 90% amino acid identity with the amino acid sequence of SEQ ID NO 2 over the entire length of the sequence of SEQ ID NO 2, or a peptide fragment thereof.
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36. A purified or isolated polypeptide selected from the group consisting of the polypeptides of SEQ ID N°
- 5 to 32 and SEQ ID 46 and 47.
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37. A method for screening ligand substances or molecules that are able to modulate the biological activity of a voltage-gated sodium channel containing a β
- 3 sub-unit, said method comprising;
(a) obtaining a recombinant host cell co-expressing a β
3 sub-unit or a fragment thereof and a functional a sub-unit, preferably an α
2 sub-unit of a voltage-gated sodium channel, or a fragment thereof;
(b) bringing into contact said recombinant host cell with a substance or molecule to be tested; and
(c) measuring an electrical parameter within the recombinant host cell brought into contact with the substance or molecule to be tested through a voltage clamp technique or measurement of membrane potential by voltage sensitive fluorescent dyes.
- 3 sub-unit, said method comprising;
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38. A method for screening ligand substances or molecules that are able to modulate the biological activity of a voltage-gated sodium channel containing a β
- 3 sub-unit, said method comprising;
(a) contacting the ligand with the β
3 sub-unit or a fragment thereof;
(b) contacting the medium containing the ligand and the β
3 protein or a fragment thereof with a β
3 substrate and allowing the possible binding of the substrate to the β
3 protein or a fragment thereof to occur; and
(c) measuring the eventual binding of the substrate to the β
3 protein or a fragment thereof.
- 3 sub-unit, said method comprising;
Specification