Method for expanding hematopoietic stem cells

• US 20040248295A1
• Filed: 04/29/2004
• Published: 12/09/2004
• Est. Priority Date: 10/30/2001
• Status: Abandoned Application

• Current Assignee: Daiichi Pharmaceutical Company Limited (Daiichi Sankyo Company Limited)
• Original Assignee: Daiichi Pharmaceutical Company Limited (Daiichi Sankyo Company Limited)
• Inventors: Hasumura, Mai, Nawa, Katsuhiko, Imada, Chiharu
• Application Number:
  US10/493,890
• Publication Number:
  US 20040248295A1
• Time in Patent Office: Days
• US Class Current: 435/372
• CPC Class Codes: C12N 2501/22 Colony stimulating factors (G-CSF, GM-CSF)
• CPC Class Codes: C12N 5/0647 Haematopoietic stem cells; Uncommitted or multipotent progenitors

Abstract

A method for culturing Lin-negative or weakly-positive cells in the presence of macrophage colony-stimulating factor (M-CSF); a method for expanding hematopoietic stem cells by culturing Lin-negative or weakly-positive cells in the presence of M-CSF; hematopoietic stem cell populations obtained by the expanding method; a M-CSF-containing kit used for culturing Lin-negative or weakly-positive cells; and a M-CSF-containing agent used for expanding hematopoietic stem cells.

Claims

1. Amethod for culturing Lin-negative or weakly-positive cells in the presence of macrophage colony-stimulating factor (M-CSF):

2. A method for expanding hematopoietic stem cells by culturing Lin-negative or weakly-positive cells in the presence of macrophage colony-stimulating factor (M-CSF).

3. A hematopoietic stem cell population obtained by the method of claim 2.

4. A kit for culturing Lin-negative or weakly-positive cells containing macrophage colony-stimulating factor (M-CSF).

5. An agent for enhancing expansion of hematopoietic stem cells containing macrophage colony-stimulating factor (M-CSF).

6. A method for culturing Lin-negative or weakly-positive cells in the presence of macrophage colony-stimulating factor (M-CSF) in order to expand hematopoietic stem cells.

7. A kit for culturing Lin-negative or weakly-positive cells containingmacrophage colony-stimulating factor (M-CSF), which is used for expansion of hematopoietic stem cells.