Method for the simultaneous and direct determination of serum cholesterol in high an low density lipoproteins using infrared spectroscopy

• US 20040248309A1
• Filed: 08/02/2004
• Published: 12/09/2004
• Est. Priority Date: 09/12/2001
• Status: Active Grant

• Current Assignee: National Research Council Canada
• Original Assignee: National Research Council Canada
• Inventors: Mantsch, Henry H., Shaw, Anthony, Liu, Kan-Zhi
• Granted Patent: US 7,022,527 B2
• Time in Patent Office: Days
• US Class Current: 436/71
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 2201/1293 resolving multicomponent spectra
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743

Abstract

A method of simultaneously determining the concentrations of cardiovascular risk markers selected from the group consisting of High Density Lipoprotein cholesterol (HDL-C), Low Density Lipoprotein cholesterol (LDL-C), total cholesterol, triglycerides and oxidized LDL using infra-red and/or near infrared light is described

Claims

1. A method of determining the concentration of at least one cardiovascular risk marker selected from the group consisting of High Density Lipoprotein cholesterol (HDL-C), Low Density Lipoprotein cholesterol (LDL-C), and oxidized LDL comprising:

subjecting a serum sample to infra-red light;

collecting at least one spectrum of infrared light after interaction with the sample; and

determining the concentration of the cardiovascular risk marker in the sample based on the intensity of spectral features or patterns over at least one spectral range selected from the group consisting of 900-1,500 cm<sup>−

1</sup>, 1500-1800 cm<sup>−

1</sup>, and 2800-3500 cm<sup>−

1</sup>.

2. The method according to claim 1 wherein the quantitative chemical analyses are derived from the spectra using a quantitation algorithm.

3. The method according to claim 2 wherein the quantitation algorithm is partial least squares analysis.

4. The method according to claim 1 wherein the sample is dried.

5. A method of determining the concentration of High Density Lipoprotein cholesterol (HDL-C) comprising:

subjecting a serum sample to infra-red light;

collecting at least one spectrum of infrared light after interaction with the sample; and

determining the concentration of HDL-C in the sample based on the intensity of spectral features or patterns over at least one spectral range selected from the group consisting of 900-1500 cm<sup>−

1</sup>, 1500-1800 cm<sup>−

1</sup>, and 2800-3500 cm<sup>−

1</sup>.

6. The method according to claim 5 wherein the quantitative chemical analyses are derived from the spectra using a quantitation algorithm.

7. The method according to claim 6 wherein the quantitation algorithm is partial least squares analysis.

8. A method of determining the concentration of Low Density Lipoprotein cholesterol (LDL-C) comprising:

subjecting a serum sample to infra-red light;

collecting at least one spectrum of infrared light after interaction with the sample;

determining the concentration of LDL-C in the sample based upon the intensity of spectral features or patterns over at least one spectral range selected from the group consisting of 1700-1800 cm<sup>−

1</sup>, and 2800-3000 cm<sup>−

1</sup>.

9. The method according to claim 8 wherein the quantitative chemical analyses are derived from the spectra using a quantitation algorithm.

10. The method according to claim 9 wherein the quantitation algorithm is partial least squares analysis.

11. A method of determining the concentration of oxidized LDL comprising:

subjecting a serum sample to infra-red light;

collecting at least one spectrum of infrared light after interaction with the sample;

quantifying the species of interest based upon the intensity of spectral features or patterns over at least one spectral range selected from the group consisting of 1500-1800 cm<sup>−

1</sup>, and 2800-3500 cm<sup>−

1</sup>.

12. The method according to claim 11 wherein the quantitative chemical analyses are derived from the spectra using a quantitation algorithm.

13. The method according to claim 11 wherein the quantitation algorithm is partial least squares analysis.