Automated microbiological testing apparatus and method

• US 20040253660A1
• Filed: 06/12/2003
• Published: 12/16/2004
• Est. Priority Date: 06/12/2003
• Status: Abandoned Application

• Current Assignee: Gilead Science Incorporated
• Original Assignee: Gilead Science Incorporated
• Inventors: Gibbs, David, Wang, Qi (Andrew)
• Application Number:
  US10/460,708
• Publication Number:
  US 20040253660A1
• Time in Patent Office: Days
• US Class Current: 435/34
• CPC Class Codes: C12Q 1/045 Culture media therefor
• CPC Class Codes: C12Q 1/18 Testing for antimicrobial activity of a material
• CPC Class Codes: G06V 20/698 Matching; Classification

Abstract

A microbiological testing method or assay for identifying an organism grown on one chromagenic semisolid nutrient media such as agar, where the organism exhibits at least one color or chromatic aspect. A digitized electrical signal is generated encoding an image of the organism on the nutrient media. The encoded image is stored and digitally processed to detect the color of the organism on the nutrient media. Chromatic characteristics of a multiplicity of known organisms are stored in an electronic library. A computer is operated to compare the detected color of the organism with chromatic characteristics stored in the library.

Claims

1. A microbiological-assay method for identifying an organism grown on one chromagenic semisolid nutrient media, said organism exhibiting at least one color on said nutrient media, said method comprising:

generating a digitized electrical signal encoding an image of said organism on said nutrient media;

storing the encoded image;

digitally processing said encoded image to detect the at least one color of said organism on said nutrient media;

storing chromatic characteristics of a multiplicity of known organisms in an electronic library; and

operating a computer to compare the detected color of said organism with chromatic characteristics stored in said library.

2. The method defined in claim 1 wherein the operating of said computer includes calculating, for each given one of a plurality of known organisms with pre-identified chromatic characteristics stored in encoded form in said library, a probability that said organism is of the same type as said given one of said known organisms.

3. The method defined in claim 2 wherein the chromatic characteristics stored in said library include, for each of said plurality of known organisms, a plurality of chromatic parameters.

4. The method defined in claim 3 wherein said chromatic parameters include at least one characteristic hue, at least one characteristic saturation, and at least one characteristic value or intensity.

5. The method defined in claim 3 wherein said chromatic parameters each include an average value and a statistical measure of variation about said average value.

6. The method defined in claim 1, further comprising preliminarily processing said encoded image to detect colonies of said organism on said nutrient media, the processing of said encoded image to detect the at least one color of said organism being performed with reference to image data pertaining to at least a selected one of the detected colonies.

7. The method defined in claim 6 wherein the preliminary processing of said encoded image includes digitally measuring at least one parameter distinguishing said colonies from said nutrient media.

8. The method defined in claim 7 wherein said at least one parameter is light intensity.

9. The method defined in claim 6 wherein the preliminary processing of said encoded image includes automatically counting the colonies, whereby a colony count is performed simultaneously on the same nutrient media as the organism identification.

10. The method defined in claim 6 wherein the processing of said encoded image includes detecting a plurality of colors each associated with a respective one of the colonies detected on said nutrient media, the operating of said computer including comparing the detected colors with chromatic characteristics stored in said library, to identify multiple organisms grown on said nutrient media.

11. The method defined in claim 1, further comprising storing in said library at least one non-chromatic optical characteristic of each of said known organisms, the digital processing of said encoded image including measuring the at least one non-chromatic optical characteristic of said organism on said nutrient media, the operating of said computer including comparing the measured non-chromatic optical characteristic of said organism with the non-chromatic optical characteristics stored in said library.

12. The method defined in claim 11 wherein said at least one non-chromatic optical characteristic is a textural characteristic.

13. The method defined in claim 12 wherein the measuring of said textural characteristic includes detecting edges in said encoded image and counting detected edges per unit area.

14. The method defined in claim 11 wherein the operating of said computer includes calculating, for each given one of a plurality of known organisms with pre-identified chromatic characteristics and pre-identified non-chromatic optical characteristics stored in encoded form in said library, a probability that said organism is of the same type as said given one of said known organisms.

15. The method defined in claim 14 wherein said chromatic characteristics and said non-chromatic optical characteristics each include an average value and a statistical measure of variation about said average value.

16. The method defined in claim 1 wherein the generating of said digitized electrical signal includes scanning said media and said organism with an optical scanner.

17. The method defined in claim 16 wherein said optical scanner is taken from the group consisting of a camera, a digital camera, and a charge-coupled device.

18. The method defined in claim 1 wherein said organism is taken from the group consisting of yeast, bacteria, and mold.

19. The method defined in claim 1 wherein said organism is a mold, further comprising:

providing said nutrient media with at least one anti-fungal composition;

depositing pieces of said mold in an array on said nutrient media provided with said anti-fungal composition;

growing said mold on said nutrient media provided with said anti-fungal composition; and

measuring effectiveness of said anti-fungal composition, the measuring of effectiveness including operating said computer to determine a size parameter of mold grown from at least one of said pieces.

20. The method defined in claim 19, further comprising inputting into said computer additional information derived from microscope observations, the operating of said computer including comparing said additional information with data stored in said library.

21. The method defined in claim 19, further comprising inputting into said computer additional information taken from the group consisting of growth rate and incubation duration, the operating of said computer including comparing said additional information with data stored in said library.

22. The method defined in claim 1, further comprising operating said computer to automatically determine an antibiotic susceptibility of said organism on said nutrient media, whereby antibiotic susceptibility and organism identification are determined simultaneously from the same plate of inoculated nutrient media.

23. The method defined in claim 22 wherein the digital processing of said encoded image includes measuring a growth-inhibition zone on said nutrient media, the operating of said computer to automatically determine the antibiotic susceptibility of said organism including determining a minimum inhibitory concentration of an antibioitic from the measurement of the growth-inhibition zone.

24. The method defined in claim 1, further comprising inputting into said computer additional information derived from microscope observations, the operating of said computer including comparing said additional information with data stored in said library.

25. The method defined in claim 1, further comprising inputting into said computer additional information taken from the group consisting of growth rate and incubation duration, the operating of said computer including comparing said additional information with data stored in said library.

26. The method defined in claim 1 wherein the processing of said encoded image includes detecting a plurality of colors each associated with a respective colony on said nutrient media, the operating of said computer including comparing the detected colors with chromatic characteristics stored in said library, to identify multiple organisms grown on said nutrient media.

27. The method defined in claim 1 wherein said nutrient media is the primary or initial agar media plate on which a patient'"'"'s specimen was first grown.

28. A microbiological-assay apparatus comprising:

a support for holding a container of chromagenic semisolid nutrient media wherein an organism of unknown identity is grown, said organism exhibiting at least one color on said nutrient media;

an optical scanning device aimed at said support for generating a digitized electrical signal encoding an image of said organism on said nutrient media;

a memory operatively connected to said scanning device for temporarily storing the encoded image;

a digital processor operatively connected to said memory for analyzing said encoded image to detect the at least one color of said organism on said nutrient media;

an electronic library storing chromatic characteristics of a multiplicity of known organisms; and

a computer operatively connected to said processor and said library, said computer being programmed to compare the detected color of said organism with chromatic characteristics stored in said library.

29. The apparatus defined in claim 28 wherein said computer is programmed to calculate, for each given one of a plurality of known organisms with pre-identified chromatic characteristics stored in encoded form in said library, a probability that said organism is of the same type as said given one of said known organisms.

30. The apparatus defined in claim 29 wherein the chromatic characteristics stored in said library include, for each of said plurality of known organisms, a plurality of chromatic parameters.

31. The apparatus defined in claim 30 wherein said chromatic parameters include at least one characteristic hue, at least one characteristic saturation, and at least one characteristic value or intensity.

32. The apparatus defined in claim 30 wherein said chromatic parameters each include an average value and a statistical measure of variation about said average value.

33. The apparatus defined in claim 28, further comprising a preprocessor operatively connected to said memory for preliminarily processing said encoded image to detect colonies of said organism on said nutrient media, said processor being operatively connected to said preprocessor to operate on image data pertaining to a selected one of the detected colonies.

34. The apparatus defined in claim 33 wherein said preprocessor includes a module for digitally measuring at least a light intensity parameter.

35. The apparatus defined in claim 28 wherein said library stores at least one non-chromatic optical characteristic of each of said known organisms, said processor including means for measuring the at least one non-chromatic optical characteristic of said organism on said nutrient media, said computer including a comparator module for comparing the measured non-chromatic optical characteristic of said organism with the non-chromatic optical characteristics stored in said library.

36. The apparatus defined in claim 35 wherein said at least one non-chromatic optical characteristic is a textural characteristic.

37. The apparatus defined in claim 36 wherein said processor includes an edge detector and an edge counter.

38. The apparatus defined in claim 35 wherein said computer includes a probability calculator for determining, for each given one of a plurality of known organisms with pre-identified chromatic characteristics and pre-identified non-chromatic optical characteristics stored in encoded form in said library, a probability that said organism is of the same type as said given one of said known organisms.

39. The apparatus defined in claim 38 wherein said chromatic characteristics and said non-chromatic optical characteristics each include an average value and a statistical measure of variation about said average value.

40. The apparatus defined in claim 28 wherein said organism is a mold, said nutrient media being provided with at least one anti-fungal composition, pieces of said mold being deposited in an array on said nutrient media provided with said anti-fungal composition, said mold being grown on said nutrient media provided with said anti-fungal composition, said computer including size detector for determining a size parameter of mold grown from at least one of said pieces.

41. The apparatus defined in claim 28 wherein said processor and said computer comprise program-modified generic digital circuits of the same electronic machine.

42. A microbiological-assay method for testing an organism grown on solid nutrient media for antibiotic susceptibility, comprising:

providing a container of semisolid nutrient media on which is disposed an organism of unknown type and an elongate strip provided at different locations with different concentrations of an antibiotic composition;

after an incubation period, optically scanning the nutrient media, said strip, and a growth region of said organism;

in response to the optical scanning, generating a digitized electrical signal encoding an image of said strip and said growth region on said nutrient media;

storing the encoded image;

digitally processing said encoded image to detect an intersection point of an edge of said growth region and said strip; and

operating a computer to determine a minimum inhibitory concentration of said antibiotic composition based on the detected intersection point.

43. The method defined in claim 42 wherein the processing of said encoded image includes operating said computer.