Methods of derivation and propagation of undifferentiated human embryonic stem (HES) cells on feeder-free matrices and human feeder layers

• US 20040253721A1
• Filed: 03/29/2004
• Published: 12/16/2004
• Est. Priority Date: 09/28/2001
• Status: Abandoned Application

• Current Assignee: ES Cell International Pte Ltd. (Lineage Cell Therapeutics, Inc.)
• Original Assignee: ES Cell International Pte Ltd. (Lineage Cell Therapeutics, Inc.)
• Inventors: Richards, Mark, Bongso, Ariffeen, Woon-Khiong, Chan, Chui-Yee, Fong
• Application Number:
  US10/811,694
• Publication Number:
  US 20040253721A1
• Time in Patent Office: Days
• US Class Current: 435/371
• CPC Class Codes: C12N 2502/13 connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
• CPC Class Codes: C12N 2502/1323 Adult fibroblasts
• CPC Class Codes: C12N 2502/243 Cells of the female genital tract, non-germinal ovarian cells
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696

Abstract

The present invention relates to the field of stem cell culture, in particular undifferentiated stem cell culture and to methods for derivation and propagation of such cells. More particularly, the invention relates to derivation and propagation of undifferentiated HES cells on human feeder layers and/or in the absence of a feeder layer. The human feeder layers may be selected from the group including human fetal muscle (HFM), human fetal skin (HFS), human adult fallopian tube (HAFT) fibroblasts and human adult skin cells. They may be cultured in the presence of a suitable medium selected from the group including Human Embryonic Stem Cell (HES), Knockout (KO), or Human Feeder (HF) medium supplemented with or without human serum.

Claims

1. A human feeder cell layer which supports the derivation of ES cells in a substantially undifferentiated state said feeder cell layer comprising cells selected from the group including human adult, fetal or embryonic cells or a combination thereof.

3. A human feeder cell layer according to claim 1 or 2 wherein the human adult cell is selected from the group including human fibroblast cells, human adult skin and human adult muscle fibroblasts and adult epithelial cells or a combination thereof.

4. A human feeder cell layer according to claim 3 wherein the human adult cell is a human fibroblast cell.

5. A human feeder cell layer according to claim 3 wherein the human fibroblast cell is a human adult fallopian tubal (HAFT) fibroblast cell.

6. A human feeder cell layer according to claim 3 wherein the human adult cell is a human skin cell.

7. A human feeder cell layer according to claim 3 wherein the human adult cell is a human muscle cell.

8. A human feeder cell layer according to claim 3 wherein the human adult cell is a human adult epithelial cell.

9. A human feeder cell layer according to claim 8 wherein the human adult epithelial cell is a human oviductal epithelial cell.

10. A human feeder cell layer according to claim 1 or 2 wherein the human fetal cell is a human fetal muscle (HFM) or human fetal skin (HFS) cell or combination thereof.

11. A human feeder cell layer according to claim 10 wherein the human fetal cell is a HFM cell.

12. A human feeder cell layer according to claim 10 wherein the human fetal cell is a HFS cell.

13. A human feeder cell layer according to claim 1 or 2 wherein the human embryonic cell is a human embryonic muscle (HEM) or human embryonic skin cell or combination thereof.

14. A human feeder cell layer according to claim 13 wherein the human embryonic cell is a HEM cell.

15. A human feeder cell layer according to claim 13 wherein the human embryonic cell is a human embryonic skin cell.

16. A human feeder cell layer according to claim 1 or 2, which is first established in a primary culture in the presence of HFE medium.

17. A human feeder cell layer according to claim 16 wherein the feeder layer is propagated in the presence of a HM medium.

18. A human feeder cell layer according to claim 1 or 2 comprising fibroblast cell line Detroit 551 (ATCC NO CCL-110).

19. A human feeder cell layer according to claim 1 or 2 comprising cell line MRC-5 having accession Number ATCC No. X-55 or ATCC No. CCL-171.

20. A human feeding layer according to claim 1 or 2 comprising cell line WI-38 having Accession Number ATCC NO CCL-75 or ATCC NO CCL-75.1.

45. A method of deriving an embryonic stem (ES) cell line in a substantially undifferentiated state from an ES cell population said method comprising:

obtaining an ES cell population comprising undifferentiated ES cells;

and culturing the undifferentiated ES cells on a cell support matrix in the presence of soluble factors derived from human feeder cells or equivalents thereof, wherein the cell support matrix comprises a human feeder cell layer according to claim 1 or 2.

46. A method according to claim 45 wherein the ES cells are cultured in the presence of a medium selected from the group including HES, KO, HES-HS, KO-HS and HF-HS as hereinbefore described.

47. A method according to claim 46 wherein the medium is KO-HS.

48. A method according to claim 45 wherein the feeder cells are first established in primary cultures in the presence of HFE medium, as hereinbefore described.

49. A method according to claim 45 wherein the feeder cells are propagated in the presence of a HM medium prior to culture with ES cells, as hereinbefore described.

50. A method according to claim 45 wherein the human feeder cell is the fibroblast cell line Detroit 551 (ATCC NO CCL-110).

51. A method according to claim 45 wherein the human feeder cell is the cell line MRC-5 having Accession Number ATCC No. X-55 or ATCC No CCL 171.

52. A method according to claim 45 wherein the human feeder cell is the cell line W1-38 having Accession Number ATCC-CCL-75 or ATCC-CCL-75.1.

53. A method according to claim 45 wherein the ES cell line is cultured in the absence of LIF.

73. A cell culture system for deriving and culturing ES cells in a substantially undifferentiated state, said culture system including:

a cell matrix; and

a cell culture medium for providing soluble factors derived from a human feeder cell selected from the group including a human adult, fetal or embryonic cell, wherein the cell support matrix comprises a human feeder cell layer according to claim 1 or 2.

76. A conditioned medium for deriving and culturing ES cell line in a substantially undifferentiated state said medium prepared by a method including:

obtaining a feeder cell layer according to claim 1 or 2;

culturing the feeder cells in the presence of a medium selected from the group including HES, KO, HES-HS, KO-HS, HFE, HM, HF, or HF-HS; and

separating the medium from the cells to obtain conditioned medium.

77. A conditioned medium according to claim 76 wherein the human feeder cell layer comprises adult skin cells.

78. A conditioned medium according to claim 77 wherein the human feeder cell layer comprises HFM cells.

79. A conditioned medium according to claim 76 wherein the medium comprises KO-HS.

2. A human feeder cell layer which supports the culture of ES cells in a substantially undifferentiated state said feeder cell layer comprising cells selected from the group including human adult, fetal or embryonic cells or a combination thereof.

21. A method of deriving an embryonic stem (ES) cell line in a substantially undifferentiated state from an ES cell population said method comprising:

obtaining an ES cell population comprising undifferentiated ES cells; and

culturing the undifferentiated ES cells on a cell support matrix in the presence of soluble factors derived from human feeder cells or equivalents thereof.

22. A method according to claim 21 wherein deriving an ES cell line is selected from the group including creating an ES cell line from a source of ES cells and wherein the ES cells are previously uncultured cells;

extending propagation or culturing time of an ES cell line wherein the ES cell line is an established cell line; and

propagating an established ES cell line.

23. A method according to claim 22 wherein the deriving of the ES cell line includes propagating an ES cell line.

24. A method according to any claim 21 wherein the ES cell population is derived from a source selected from the group including an embryo, blastocyst, inner cell mass (ICM) cells, and a culture of ES cells which have not differentiated.

25. A method according to claim 24 wherein the source is from a blastocyst.

26. A method according to claim 21 wherein the soluble factors are derived from human feeder cells selected from the group including human adult, fetal or embryonic cells or a combination thereof.

27. A method according to claim 26 wherein the human adult cell is selected from the group including human fibroblast cells, human adult skin and human adult muscle fibroblasts and adult epithelial cells or a combination thereof.

28. A method according to claim 27 wherein the human adult cell is a human fibroblast cell.

29. A method according to claim 27 wherein the human fibroblast cell is a human adult fallopian tubal (HAFT) fibroblast cell.

30. A method according to claim 27 wherein the human adult cell is a human skin cell.

31. A method according to claim 27 wherein the human adult cell is a human muscle cell.

32. A method according to claim 27 wherein the human adult cell is a human adult epithelial cell.

33. A method according to claim 27 wherein the human adult cell is a human oviductal epithelial fibroblast.

34. A method according to claim 26 wherein the human fetal cell is a human fetal muscle (HFM) or human fetal skin (HFS) cell or combination thereof.

35. A method according to claim 34 wherein the human fetal cell is a HFM cell.

36. A method according to claim 34 wherein the human fetal cell is a HFS cell.

37. A method according to claim 26 wherein the human embryonic cell is a human embryonic muscle (HEM) or human embryonic skin cell or combination thereof.

38. A method according to claim 37 wherein the human embryonic cell is a HEM cell.

39. A method according to claim 37 wherein the human embryonic cell is a human embryonic skin cell.

40. A method according to claim 21 wherein the human feeder cells are cultured in the presence of a medium selected from a group including HES, KO, HF, HES-HS, KO-HS, and HF-HS as hereinbefore described.

41. A method according to claim 40 wherein the medium is HES-HS or KO-HS.

42. A method according to claim 41 wherein the medium is KO-HS.

43. A method according to claim 21 wherein the cell support matrix is a non-cellular cell support matrix selected from the group including Collagen I, Collagen IV, human extracellular matrix or Matrigel or a combination thereof.

44. A method according to claim 43 wherein the cell support matrix comprises Collagen I or Type I Collagen.

54. A cellular composition comprising proliferating undifferentiated ES cells and wherein the cell composition comprises the propagated or derived ES cells prepared by the methods according to claim 21.

55. An undifferentiated ES cell line prepared by a method according to claim 21.

56. A cell culture system for deriving and culturing ES cells in a substantially undifferentiated state, said culture system including:

a cell support matrix; and

a cell culture medium for providing soluble factors derived from a human feeder cell selected from the group including a human adult, fetal or embryonic cell.

57. A cell culture system according to claim 56 wherein the human adult cell is selected from the group including human adult fallopian tubal (HAFT) fibroblast cells, human adult skin and human adult muscle fibroblasts and adult epithelial cells or a combination thereof.

58. A cell culture system according to claim 57 wherein the human adult cell is a human fibroblast cell.

59. A cell culture system according to claim 57 wherein the human adult cell is a human adult fallopian tubal (HAFT) fibroblast cell.

60. A cell culture system according to claim 57 wherein the human adult cell is a human skin cell.

61. A cell culture system according to claim 57 wherein the human adult cell is a human muscle cell.

62. A cell culture system according to claim 57 wherein the human adult cell is a human adult epithelial cell.

63. A cell culture system according to claim 57 wherein the human epithelial adult cell is a human oviductal epithelial cell.

64. A cell culture system according to claim 56 wherein the human fetal cell is a human fetal muscle (HFM) or human fetal skin (HFS) cell or combination thereof.

65. A cell culture system according to claim 64 wherein the human fetal cell is a HFM cell.

66. A cell structure system according to claim 64 wherein the human fetal cell is a HFS cell.

70. A cell culture system according to claim 56 wherein the cell support matrix comprises Collagen I or matrigel or a combination thereof.

71. A cell culture system according to claim 70 wherein the cell support matrix comprises Collagen I.

72. A cell culture system according to claim 56 wherein the cell culture medium is a conditioned medium including soluble factors derived from a human feeder cell layer.

74. A cell culture system according to claim 56 wherein the culture medium is selected from the group including HES, KO, HES-HS, and KO-HS.

75. A cell culture system according to claim 74 wherein the medium is KO-HS.

67. A cell culture system according to 56 wherein the human embryonic cell is a human embryonic muscle (HEM) or human embryonic skin cell or combination thereof.

68. A cell culture system according to claim 67 wherein the human embryonic cell is a HEM cell.

69. A cell culture system according to claim 67 wherein the human embryonic cell is a human embryonic skin cell.