Antisense modulation of FLIP-c expression

• US 20040254137A1
• Filed: 10/27/2003
• Published: 12/16/2004
• Est. Priority Date: 09/20/2000
• Status: Abandoned Application

• Current Assignee: Andrew T. Watt, C. Frank Bennett, Elizabeth J. Ackermann, Hong Zhang, Nicholas M. Dean, William Ricketts
• Original Assignee: Andrew T. Watt, C. Frank Bennett, Elizabeth J. Ackermann, Hong Zhang, Nicholas M. Dean, William Ricketts
• Inventors: Watt, Andrew T., Dean, Nicholas M., Zhang, Hong, Bennett, C. Frank, Ackermann, Elizabeth J., Ricketts, William
• Application Number:
  US10/695,568
• Publication Number:
  US 20040254137A1
• Time in Patent Office: Days
• US Class Current: 514/44
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/315 Phosphorothioates
• CPC Class Codes: C12N 2310/321 2'-O-R Modification
• CPC Class Codes: C12N 2310/3341 5-Methylcytosine
• CPC Class Codes: C12N 2310/341 Gapmers, i.e. of the type ===---===
• CPC Class Codes: C12N 2310/346 having a combination of backbone and sugar modifications
• CPC Class Codes: C12N 2310/3525 MOE, methoxyethoxy
• CPC Class Codes: Y02P 20/582 Recycling of unreacted starting or intermediate materials

Abstract

Antisense compounds, compositions and methods are provided for modulating the expression of FLIP-c. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding FLIP-c. Methods of using these compounds for modulation of FLIP-c expression and for treatment of diseases associated with expression of FLIP-c are provided.

Claims

1-20. -20. (canceled).

21. A method for activating a caspase signaling cascade in a cell comprising contacting a cell with an inhibitor of FLIP-c so that the at least one caspase is cleaved thereby indicating activation of a caspase signaling cascade.

22. The method of claim 21, wherein the inhibitor of FLIP-c is a compound 15 to 25 nucleobases in length targeted to a nucleic acid molecule encoding FLIP-c, wherein said compound specifically hybridizes with and inhibits the expression of FLIP-c.

23. The compound of claim 22 which is an antisense oligonucleotide.

24. The compound of claim 23, wherein the antisense oligonucleotide is chimeric or comprises at least one modified internucleoside linkage, sugar moiety, or nucleobase.

25. The method of claim 21, wherein the caspase is caspase 3, caspase 7 or caspase 8.

26. The method of claim 21, further comprising contacting the cell with TRAIL.