Needle punched textile for use in growing anatomical elements
First Claim
1. A system for generating tissue using a scaffold at least partially made up of needle punched textile.
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Abstract
In a system for generating tissue by growing cells in a porous and sometimes biodegradable material, a needle punched textile which serves as a scaffold is used for growing any of a variety of anatomical elements, in which the thickness of areas of the anatomical element and thus its strength can be increased by providing layers of mesh which are needled together to form a layerless textile and in which delamination is prevented through the use of the needling. In one embodiment, the needle punched textile is utilized to form a semi-lunar heart valve. In a preferred embodiment for pediatric use, the textile is made from two different biodegradable non-woven meshes. For some adult applications biodegradable meshes are not necessary, thus eliminating the necessity of using two different needled meshes. In one embodiment the needling is done with a single needle which is made to move around the periphery of a mold used in making the scaffold, thus to precisely control the area needled. Also a method for culturing cell-scaffold construction is described.
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Citations
34 Claims
- 1. A system for generating tissue using a scaffold at least partially made up of needle punched textile.
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7. A system for varying the biodegradability of a scaffold used in tissue engineering, comprising:
a number of different layers of mesh each having a different biodegradability, said layers being needle punched together. - View Dependent Claims (8)
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9. A method of forming a scaffold for tissue generation, comprising:
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providing a number of layers of non-woven mesh and a mold; and
,needling around the periphery of the mold so as to join portions of the layers at the periphery of the mold. - View Dependent Claims (10, 11, 12)
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13. A method of forming a uniform thickness of scaffold for growing tissue to form anatomical parts, comprising:
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making a least a portion of the scaffold from two layers of non-woven mesh; and
,needling the layers together to form a uniform thickness layerless textile.
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14. A method of culturing cell-scaffold constructs for the production of substitute anatomical elements, comprising the steps of:
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putting cells onto the scaffold using a hybridization tube until some cells attach to the scaffold;
placing the scaffold into a roller bottle;
placing culture medium in a roller bottle; and
,rotating the roller bottle in an incubator to proliferate the cells and for the cells to lay down extra cellular matrix. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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29. A method for constructing a synthetic heart valve, comprising the steps of:
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forming a tissue engineered heart valve using needle punched textile layers; and
implanting the valve. - View Dependent Claims (30, 31, 32, 33)
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34. An implantable pediatric heart valve comprising:
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a biodegradable scaffold defining a valve geometry and including layers of textile mesh needle punched together in selected areas; and
,cells grown on said scaffold to form said valve, said-scaffold degrading over time to leave in place a valve made of cells and an extra cellular matrix.
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Specification