Post-conditioning for the reduction of ischemic-reperfusion injury in the heart and other organs

• US 20040255956A1
• Filed: 06/16/2004
• Published: 12/23/2004
• Est. Priority Date: 12/21/2001
• Status: Abandoned Application

• Current Assignee: Emory University
• Original Assignee: Emory University
• Inventors: Vinten-Johansen, Jakob, Zhao, Zhi-Qing
• Application Number:
  US10/499,052
• Publication Number:
  US 20040255956A1
• Time in Patent Office: Days
• US Class Current: 128/898
• CPC Class Codes: A61M 1/3613 Reperfusion, e.g. of the coronary vessels, e.g. retroperfusion
• CPC Class Codes: A61M 1/3621 Extra-corporeal blood circuits single-needle circuits A61M1/30
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system

Abstract

The present invention provides a method of post-conditioning reperfusion of an organ or tissue injured by ischemia. Also provided is a method of treating a myocardial infarction in a subject to prevent injury to the heart following reperfusion of the heart.

Claims

1. A method of preventing injury to an organ or tissue in a subject during or after reperfusion following an ischemic event to the organ or tissue, comprising the following steps:

a) stopping perfusion of the organ for from about 5 seconds to about 5 minutes;

b) resuming perfusion of the organ for from about 5 seconds to about 5 minutes;

c) repeating steps a) and b) sequentially for from about 2 to about 50 times; and

d) allowing uninterrupted perfusion of the organ or tissue, thereby preventing injury to the organ or tissue in the subject following an ischemic event.

2. The method of claim 1, wherein the organ or tissue is heart, brain, kidney, intestine, pancreas, liver, lung or skeletal muscle.

3. The method of claim 1, wherein the subject is a mammal.

4. The method of claim 3, wherein the mammal is a human.

5. The method of claim 1, wherein stopping perfusion is effected by a balloon within a lumen of a blood vessel that supplies blood to the organ or tissue.

6. The method of claim 5, wherein the balloon is inflatable and deflatable.

7. The method of claim 1, wherein stopping perfusion is effected by external compression of a blood vessel that supplies blood to the organ or tissue.

8. The method of claim 1, further comprising administering to the subject an effective amount of a tissue protective agent in a pharmaceutically acceptable carrier.

9. A method of preventing injury to a heart in a subject diagnosed with an ischemic event of the heart, comprising:

a) clearing a lumen of a coronary artery;

b) perfusing the heart for from about 5 seconds to about 5 minutes;

c) stopping perfusion of the heart for from about 5 seconds to about 5 minutes;

d) repeating steps b) and c) sequentially for from about 2 to about 50 times; and

e) allowing uninterrupted perfusion of the heart, thereby preventing injury to the heart in the subject.

10. The method of claim 9, wherein stopping perfusion is effected by a balloon within a lumen of the coronary artery.

11. The method of claim 10, wherein the balloon is inflatable and deflatable.

12. The method of claim 9, wherein stopping perfusion is effected by external compression of the coronary artery.

13. The method of claim 9, further comprising administering to the subject an effective amount of a tissue protective agent in a pharmaceutically acceptable carrier.

14. A method of preventing injury to an organ or tissue in a subject during or after reperfusion following an ischemic event to the organ or tissue, comprising the following steps:

a) reducing perfusion of the organ for from about 5 seconds to about 5 minutes;

b) resuming perfusion of the organ for from about 5 seconds to about 5 minutes;

c) repeating steps a) and b) sequentially for from about 2 to about 50 times; and

d) allowing uninterrupted perfusion of the organ or tissue, thereby preventing injury to the organ or tissue in the subject following an ischemic event.

15. The method of claim 14, wherein the organ or tissue is heart, brain, kidney, intestine, pancreas, liver, lung or skeletal muscle.

16. The method of claim 14, wherein the subject is a mammal.

17. The method of claim 16, wherein the mammal is a human.

18. The method of claim 14, wherein reducing perfusion is effected by a balloon within a lumen of a blood vessel that supplies blood to the organ or tissue.

19. The method of claim 18, wherein the balloon is inflatable and deflatable.

20. The method of claim 14, wherein reducing perfusion is effected by external compression of a blood vessel that supplies blood to the organ or tissue.

21. The method of claim 14, further comprising administering to the subject an effective amount of a tissue protective agent in a pharmaceutically acceptable carrier.

22. A method of preventing injury to a heart in a subject diagnosed with an ischemic event of the heart, comprising:

a) clearing a lumen of a coronary artery;

b) perfusing the heart for from about 5 seconds to about 5 minutes;

c) reducing perfusion of the heart for from about 5 seconds to about 5 minutes;

d) repeating steps b) and c) sequentially for from about 2 to about 50 times; and

e) allowing uninterrupted perfusion of the heart, thereby preventing injury to the heart in the subject.

23. The method of claim 22, wherein reducing perfusion is effected by a balloon within a lumen of the coronary artery.

24. The method of claim 23, wherein the balloon is inflatable and deflatable.

25. The method of claim 22, wherein reducing perfusion is effected by external compression of the coronary artery.

26. The method of claim 22, further comprising administering to the subject an effective amount of a tissue protective agent in a pharmaceutically acceptable carrier.