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Hairpin primer amplification

  • US 20040259116A1
  • Filed: 01/27/2004
  • Published: 12/23/2004
  • Est. Priority Date: 01/28/2003
  • Status: Abandoned Application
First Claim
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1. A method for amplifying a target nucleotide sequence or reverse complement thereof, the method comprising:

  • providing a template nucleic acid comprising a first strand, the first strand comprising a target region that comprises the target nucleotide sequence or its reverse complement;

    providing a first linear primer comprising a region of identity to a 5′

    subregion of the target region;

    providing a second linear primer comprising a region of complementarity to a 3′

    subregion of the target region;

    providing a hairpin primer comprising a region of complementarity to a first subregion of the target region, the first subregion being 5′

    of or at least partially overlapping the 3′

    subregion;

    contacting the template nucleic acid, the first linear primer, the second linear primer and the hairpin primer; and

    , extending at least the hairpin primer, thereby amplifying at least a portion of the target nucleotide sequence or its reverse complement.

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