Glycerol as a predictor of glucose tolerance

• US 20040259134A1
• Filed: 04/19/2004
• Published: 12/23/2004
• Est. Priority Date: 10/22/1999
• Status: Abandoned Application

• Current Assignee: Complexe Hospitalier De La Sagamie, Whitehead Institute for Biomedical Research
• Original Assignee: Complexe Hospitalier De La Sagamie, Whitehead Institute for Biomedical Research
• Inventors: Hudson, Thomas J., Daly, Mark J., Arsenault, Steve, Rioux, John D., Gaudet, Daniel
• Application Number:
  US10/827,131
• Publication Number:
  US 20040259134A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
• CPC Class Codes: C12Q 1/48 involving transferase
• CPC Class Codes: C12Q 1/485 involving kinase
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Y 207/0103 Glycerol kinase (2.7.1.30)
• CPC Class Codes: G01N 2333/91215 with a definite EC number (2.7.1.-)
• CPC Class Codes: G01N 2500/00 Screening for compounds of potential therapeutic value
• CPC Class Codes: G01N 2800/042 Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
• CPC Class Codes: G01N 2800/52 Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere

Abstract

Novel alterations in the glycerol kinase gene are described. Also described are methods of predicting or assisting in the prediction of impaired glucose tolerance and type 2 diabetes mellitus.

Claims

1. An isolated nucleic acid molecule comprising a portion of SEQ ID NO:

3, wherein said portion is at least 10 nucleotides in length and includes nucleotide position 29 of exon 10 of a glycerol kinase (GK) gene, and wherein said nucleic acid molecule comprises a mutant allele of said GK gene at said nucleotide position 29.

3. The nucleic acid molecule of claim 1, wherein said portion is at least 20 nucleotides in length.

4. The nucleic acid molecule of claim 1, wherein said portion is at least 50 nucleotides in length.

5. The nucleic acid molecule of claim 1, wherein said mutant allele comprises a nucleotide selected from the group consisting of a guanosine, a thymidine, and a cytidine at said nucleotide position 29.

6. The nucleic acid molecule of claim 5, wherein said nucleic acid molecule comprises a guanosine at said nucleotide position 29.

7. The nucleic acid molecule of claim 1, wherein the nucleic acid molecule is selected from the group consisting of DNA, RNA, and polypeptide nucleic acid (PNA).

8. The nucleic acid molecule of claim 1, wherein the nucleic acid molecule comprises a label.

9. The nucleic acid molecule of claim 8, wherein the label is selected from the group consisting of a radioisotope, a fluorescent compound, an enzyme, and an enzyme cofactor.

10. The nucleic acid molecule of claim 1, wherein the nucleic acid molecule is immobilized on a solid support.

11. The nucleic acid molecule of claim 10, wherein the nucleic acid molecule is one of an array of two or more different nucleic acid molecules immobilized on said solid support.

2. A complement strand of a nucleic acid molecule, wherein said nucleic acid molecule comprises a portion of SEQ ID NO:

3, wherein said portion is at least 10 nucleotides in length and includes nucleotide position 29 of exon 10 of a glycerol kinase (GK) gene, and wherein said nucleic acid molecule comprises a mutant allele of said GK gene at said nucleotide position 29.

12. An isolated nucleic acid molecule comprising SEQ ID NO:

3, wherein said nucleic acid molecule comprises a mutant allele at nucleotide position 29 of exon 10 of a glycerol kinase (GK) gene.

14. The nucleic acid molecule of claim 12, wherein said nucleic acid molecule is at least 250 nucleotides in length.

15. The nucleic acid molecule of claim 12, wherein said mutant allele comprises a nucleotide selected from the group consisting of a guanosine, a thymidine, and a cytidine at said nucleotide position 29.

16. The nucleic acid molecule of claim 15, wherein said nucleic acid molecule comprises a guanosine at said nucleotide position 29.

17. The nucleic acid molecule of claim 12, wherein the nucleic acid molecule is selected from the group consisting of DNA, RNA, and polypeptide nucleic acid (PNA).

18. The nucleic acid molecule of claim 12, wherein the nucleic acid molecule comprises a label.

19. The nucleic acid molecule of claim 18, wherein the label is selected from the group consisting of a radioisotope, a fluorescent compound, an enzyme, and an enzyme cofactor.

20. The nucleic acid molecule of claim 12, wherein the nucleic acid molecule is immobilized on a solid support.

21. The nucleic acid molecule of claim 20, wherein the nucleic acid molecule is one of an array of two or more different nucleic acid molecules immobilized on said solid support.

13. A complement strand of a nucleic acid molecule, wherein said nucleic acid molecule comprises SEQ ID NO:

3, wherein said nucleic acid molecule comprises a mutant allele at nucleotide position 29 of exon 10 of a glycerol kinase (GK) gene.

22. An isolated nucleic acid molecule which specifically hybridizes to a portion of SEQ ID NO:

3, wherein said portion is at least 10 nucleotides in length and includes nucleotide position 29 of exon 10 of a glycerol kinase (GK) gene, and wherein said nucleic acid molecule comprises a mutant allele of said GK gene at said nucleotide position 29.

23. An isolated nucleic acid molecule consisting of a portion of SEQ ID NO:

3, wherein said portion is at least 10 nucleotides in length and includes nucleotide position 29 of exon 10 of a glycerol kinase (GK) gene, and wherein said nucleic acid molecule comprises a mutant allele of said GK gene at said nucleotide position 29.

24. An isolated nucleic acid molecule consisting of SEQ ID NO:

3, wherein said nucleic acid molecule comprises a mutant allele at nucleotide position 29 of exon 10 of a glycerol kinase (GK) gene at said nucleotide position 29.