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Methods, compositions, and growth and differentiation factors for insulin-producing cells

  • US 20040259244A1
  • Filed: 03/15/2004
  • Published: 12/23/2004
  • Est. Priority Date: 05/28/2002
  • Status: Abandoned Application
First Claim
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1. A method of converting differentiated non-hormone producing pancreatic cells into differentiated hormone-producing cells, comprising:

  • a) culturing said differentiated non-hormone producing pancreatic cells in a first cell culture system with a first cell culture medium comprising a basal medium, with or without serum, and with or without growth factors;

    under conditions which provide for converting said differentiated non-hormone producing pancreatic cells into stem cells; and

    b) culturing said stem cells in a second cell culture system with a second cell culture medium comprising at least one compound selected from Group A, wherein the compounds of Group A are selected from the group consisting of;

    Betacellulin, Activin A, BMP-2, TGF-β

    SRII, DMSO, Sonic Hedgehog, Laminin, Met-Enkephalin, DMF, and Cholera Toxin A;

    and at least one compound selected from Group B, wherein the compounds of Group B are selected from the group consisting of;

    Activin A, Atrial Natriuretic Peptide, Betacellulin, Bone Morphogenic Protein (BMP-2), Bone Morphogenic Protein (BMP-4), C natriuretic peptide (CNP), Caerulein, Calcitonin Gene Related Peptide (CGRP-ax), Cholecystokinin (CCK8-amide), Cholecystokinin octapeptide (CCK8-sulfated), Cholera Toxin B Subunit, Corticosterone (Reichstein'"'"'s substance H), Dexamethasone, DIF-1, Differanisole A, Dimethylsulfoxide (DMSO), EGF, Endothelin 1, Exendin 4, FGF acidic, FGF2, FGF7, FGFb, Gastrin I, Gastrin Releasing Peptide (GRP), Glucagon-Like Peptide 1 (GLP-1), Glucose, Growth Hormone, Hepatocyte Growth Factor (HGF), IGF-1, IGF-2, Insulin, KGF, Lactogen, Laminin, Leu-Enkephalin, Leukemia Inhibitory Factor (LIF), Met-Enkephalin, n Butyric Acid, Nerve Growth Factor (β

    -NGF), Nicotinamide, n-n-dimethylformamide (DMF), Parathyroid Hormone Related Peptide (Pth II RP), PDGF AA+PDGF BB MIX, PIGF (Placental GF), Progesterone, Prolactin, Putrescine Dihydrochloride Gamma-Irradiated Cell Culture, REG1, Retinoic Acid, Selenium, Selenious Acid, Sonic Hedgehog, Soybean Trypsin Inhibitor, Substance P, Superoxide Dismutase (SOD), TGF-α

    , TGF-β

    sRII, TGF-β

    1, transferrin, Triiodothyronine (T3), Trolox, Vasoactive Intestinal Peptide (VIP), VEGF, Vitamin A, and Vitamin E;

    under conditions which provide for differentiating said stem cells into hormone-producing cells.

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