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Optical biosensor with enhanced activity retention for detection of halogenated organic compounds

  • US 20040265811A1
  • Filed: 08/09/2004
  • Published: 12/30/2004
  • Est. Priority Date: 06/01/2001
  • Status: Active Grant
First Claim
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1. A distal tip of a biosensor ion sensing transducer for use in detecting an analyte comprising an halogenated organic compound in an environment, the tip comprising:

  • a biocomponent comprising at least one enzyme selected from the group consisting of hydrolases, subclass EC 3.8, and lyases, subclass EC 4.5, for carrying out a dehalogenation of the compound, said biocomponent immobilized to a surface of the tip by means selected from the group consisting of (a) entrapment within a hydrogel;

    (b) entrapment within a polymeric network;

    (c) (micro)encapsulation;

    (d) covalent-bonding;

    and (e) adsorption;

    a treatment of said biocomponent for maintaining a period of enzymatic efficacy; and

    said biocomponent stabilized by means selected from the group consisting of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component of said immobilizing means.

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