Sets of generalized target-binding e-tag probes
0 Assignments
0 Petitions
Accused Products
Abstract
Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
-
Citations
30 Claims
-
1-20. -20. (Canceled)
-
21. A probe set for detecting the presence or absence of one or more target compounds, the probe set comprising a plurality of electrophoretic probes selected from the group defined by the formula:
-
[(D, M)-L]k-T wherein;
T is a target-binding moiety specific for a target compound;
k is an integer in the range of from 1 to 20;
L is a cleavable linkage;
D is a detection group;
M is a mobility modifier consisting of from 1 to 500 atoms selected from the group consisting of carbon, hydrogen, oxygen, sulfur, nitrogen, phosphorus, and boron; and
wherein, upon cleavage of L an eTag reporter comprising a detection group, D, and a mobility modifier, M, is produced with a distinct charge/mass ratio so that eTag reporters from different electrophoretic probes form distinct peaks upon electrophoretic separation.- View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30)
-
Specification