Genomic and proteomic approaches for the development of cell culture medium
First Claim
1. A method for formulating a cell culture medium comprising:
- detecting a sequence in a cell, the sequence being a nucleic acid sequence or an expressed amino acid sequence, and formulating a cell culture medium to contain a molecule to modulate the detected sequence or its expression or to modulate a cellular process affected by the detected sequence or its expression.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a method for designing a cell culture medium adapted to support a cell line in a pre-defined manner. The method includes generating an expression profile of a cell line, identifying from the expression profile a set of biomolecules to evaluate for their effect on an end-point assay using the cell line, testing each biomolecule in the set for its effect in the endpoint assay, wherein each biomolecule that is determined to have a measurable effect in the end-point assay relative to a control, not containing the biomolecule, is considered a positive biomolecule and formulating a cell culture medium for the cell line by adding a positive biomolecule to a basal medium to form a modified medium, and determining whether the modified medium is sufficient to support the cell line in the pre-defined manner. Methods for identifying biomolecules for use in designing a cell culture medium adapted to support a cell line in a pre-defined manner, methods for preparing a serum-free cell culture medium that is sufficient to support a cell line and arrays for use in such methods are also provided.
-
Citations
77 Claims
-
1. A method for formulating a cell culture medium comprising:
-
detecting a sequence in a cell, the sequence being a nucleic acid sequence or an expressed amino acid sequence, and formulating a cell culture medium to contain a molecule to modulate the detected sequence or its expression or to modulate a cellular process affected by the detected sequence or its expression. - View Dependent Claims (3)
-
-
2. A method for formulating a cell culture medium, said method comprising:
-
detecting a sequence in a cell, the sequence being a nucleic acid sequence or an expressed amino acid sequence;
determining whether a molecule modulates the sequence or its expression or modulates a cellular process affected by the sequence or its expression;
formulating a cell culture medium to contain a molecule to modulate the detected sequence or its expression or to modulate a cellular process affected by the detected sequence or its expression. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
-
-
25. A method for preparing a cell culture medium, said method comprising:
-
contacting a polynucleotide with an array comprising a plurality of biopolymers immobilized on the surface of the array, the polynucleotide being derived from a cell or the complement thereof;
detecting a bound pair formed between the polynucleotide and an immobilized biopolymer;
selecting a molecule for inclusion in a cell culture medium based on the members of the detected bound pair;
testing selected molecule to determine its effect on a cellular process of the cell; and
formulating a cell culture medium to include the selected molecule. - View Dependent Claims (26, 28, 29)
-
-
27. A method for preparing a cell culture medium, said method comprising:
-
contacting a polypeptide with an array comprising a plurality of biopolymers immobilized on the surface of the array;
detecting a bound pair formed between the polypeptide and an immobilized biopolymer;
selecting a molecule for inclusion in a cell culture medium based on the members of the detected bound pair;
testing selected molecule to determine its effect on a cellular process of the cell; and
; and
formulating a cell culture medium to include the selected molecule.
-
- 30. An array comprising at least one nucleic acid of, but less than the entire genome of, a Chinese hamster cell, said nucleic acid being immobilized on the surface of the array.
-
35. A method for designing a cell culture medium adapted to support a cell line in a pre-defined manner, which method comprises:
-
(a) generating an expression profile of a cell line;
(b) identifying from the expression profile a set of biomolecules to evaluate for their effect on an end-point assay using the cell line;
(c) testing each biomolecule in the set for its effect in the endpoint assay, wherein each biomolecule that is determined to have a measurable effect in the end-point assay relative to a control, not containing the biomolecule, is considered a positive biomolecule; and
(d) formulating a cell culture medium for the cell line by adding a positive biomolecule to a basal medium to form a modified medium, and determining whether the modified medium is sufficient to support the cell line in the pre-defined manner. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 69)
-
-
67. A method for identifying biomolecules for use in designing a cell culture medium adapted to support a cell line in a pre-defined manner comprising:
-
(a) generating a pool of polynucleotide probes that are complementary to polynucleotide sequences that encode fragments of polypeptides expressed by a cell line;
(b) contacting the pool of polynucleotide probes under hybridizing conditions with at least one array comprising a plurality of biopolymers immobilized on the surface of the array, each biopolymer encoding a fragment of a distinct polypeptide that participates in a biologically significant cellular process;
(c) generating an expression profile by detecting each polynucleotide probe that hybridizes to each biopolymer; and
(d) selecting biomolecules, based on the expression profile, to be candidate components for a cell culture medium based on the function of the polypeptide partially encoded by the biopolymers to which a probe hybridizes. - View Dependent Claims (70)
-
-
68. A method for preparing a serum-free cell culture medium that is sufficient to support a cell line, which method comprises:
-
(a) generating a pool of polynucleotide probes that are complementary to polynucleotide sequences that encode fragments of polypeptides expressed by a cell line;
(b) contacting the pool of polynucleotide probes under hybridizing conditions with at least one array comprising a plurality of biopolymers immobilized on the surface of the array, each biopolymer encoding a fragment of a distinct polypeptide that participates in a biologically significant cellular process;
(c) generating an expression profile by detecting each polynucleotide probe that hybridizes to each biopolymer;
(d) selecting biomolecules, based on the expression profile, to be candidate components for a serum-free medium based on the function of the polypeptide partially encoded by the biopolymers to which a probe hybridizes;
(e) testing each candidate component to determine its effect on growth and/or proliferation of the cell line, and designating those candidate components that increase growth and/or proliferation of the cell line as positive biomolecules; and
(f) adding a positive biomolecule to a serum-free basal medium and evaluating the growth and/or proliferation of the cell line in the modified medium.
-
- 71. An array for designing and/or optimizing cell culture medium, which comprises a pool of biopolymers immobilized on a surface of a substrate, each biopolymer selected from the group consisting of polynucleotides encoding fragments of distinct polypeptides that are participants in biologically significant cellular processes, antibodies or antibody fragments that specifically bind to polypeptides that are participants in biologically significant cellular processes and fragments of polypeptides that are participants in biologically significant cellular processes.
Specification