Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting AP-1 transcription factors
First Claim
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1. A bead-based method for identifying both thioaptamer sequences and binding proteins comprising the steps of:
- incubating a thioaptamer library with a sample suspected of comprising one or more proteins;
selecting the one or more thioaptamers of the library to which protein has bound;
identifying the proteins by mass spectrometry; and
identifying the thioaptamer by PCR, cloning and sequencing.
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Abstract
The present invention includes composition and methods for making and using a combinatorial library to identify modified thioaptamers that bind to, and affect the immune response of a host animal, transcription factors such as IL-6, NF-κB, AP-1 and the like. Composition and methods are also provided for the treatment of viral infections, as well as, vaccines and vaccine adjuvants are provided that modify host immune responses.
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45 Claims
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1. A bead-based method for identifying both thioaptamer sequences and binding proteins comprising the steps of:
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incubating a thioaptamer library with a sample suspected of comprising one or more proteins;
selecting the one or more thioaptamers of the library to which protein has bound;
identifying the proteins by mass spectrometry; and
identifying the thioaptamer by PCR, cloning and sequencing. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A system for identifying proteins that interact with a specific thioaptamer comprising:
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a thioaptamer library for binding one or more proteins;
a bead separator wherein the one or more thioaptamer beads of the library to which protein has bound are separated;
a mass spectrometer to identify the proteins that have bound to the one or thioaptamer beads. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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43. A bead-based method for identifying one or more proteins that bind to one or more thioaptamers comprising the steps of:
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incubating a thioaptamer library with a sample suspected of comprising one or more proteins; and
identifying the proteins by mass spectrometry.
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44. A method for identifying one or more targets that bind to one or more thioaptamers comprising the steps of:
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incubating a thioaptamer library with a sample suspected of comprising one or more targets; and
identifying the target by mass spectrometry. - View Dependent Claims (45)
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Specification