Methods for correcting mitotic spindle defects associated with somatic cell nuclear transfer in animals
First Claim
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1. A method comprising the steps of:
- introducing nuclei along with one or more molecular components into an egg;
culturing said egg to produce a viable embryo;
transferring said embryo to the oviducts of a female; and
producing a cloned animal.
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Abstract
The present invention is directed to various methodologies to make NT a practical procedure for animals, specifically, primates including human and non-human primates. Furthermore, the methods and molecular components provided by the present invention provide a practical means for producing embryos with desired characteristics. In a specific embodiment, the methodology of the present invention comprises introducing nuclei having desired characteristics along with one or more molecular components into an egg, culturing the egg to produce a viable embryo, transferring the embryo to the oviducts of a female, and producing a cloned animal.
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Citations
84 Claims
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1. A method comprising the steps of:
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introducing nuclei along with one or more molecular components into an egg;
culturing said egg to produce a viable embryo;
transferring said embryo to the oviducts of a female; and
producing a cloned animal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method comprising the steps of:
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introducing nuclei along with one or more molecular components into an egg;
culturing said egg to produce a viable embryo;
dissociating blastomeres from said embryo; and
culturing said blastomeres to produce stem cells. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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50. A method comprising the steps of:
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introducing nuclei along with one or more molecular components into an egg;
culturing said egg to produce a viable embryo; and
transferring said embryo to the oviducts of a female. - View Dependent Claims (51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68)
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69. A method comprising the steps of:
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introducing nuclei along with one or more molecular components into an egg;
culturing said egg to produce a viable embryo; and
dissociating blastomeres from said embryo. - View Dependent Claims (70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84)
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Specification