Target analyte detection using asymmetrical self-assembled monolayers

• US 20050003398A1
• Filed: 04/12/2004
• Published: 01/06/2005
• Est. Priority Date: 05/01/2000
• Status: Abandoned Application

• Current Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Original Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Inventors: Tao, Chunlin, Yu, Changjun
• Application Number:
  US10/823,502
• Publication Number:
  US 20050003398A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 2610/00 Assays involving self-assembled monolayers [SAMs]
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/553 Metal or metal coated

Abstract

The present invention relates to the use asymmetric monolayer forming species and electroconduit forming species to detect target analytes.

Claims

1. A method of modifying a metallic surface comprising contacting the metallic surface with an asymmetric monolayer forming species having the formula:

2. A method according to claim 1 further comprising contacting said metallic surface with a biological species having the formula:

A-MFS-capture binding ligand

wherein

A is an attachment linker; and

MFS is a monolayer forming species.

3. A method according to claim 2 wherein said capture binding ligand is a nucleic acid.

4. A method according to claim 2 wherein said capture binding ligand is a n protein.

5. A method according to claim 1 wherein A is sulfur.

6. A method according to claim 1 wherein said metallic surface is gold.

7. A method according to claim 1 wherein said MFS is an insulator.

8. A method according to claim 7 wherein said insulator comprises an alkyl group from about 7 to 20 carbons.

9. A method according to claim 8 wherein said alkyl group comprises a heteroalkyl.

10. A method according to claim 8 wherein said alkyl group comprises a substituted alkyl.

11. A method according to claim 1 wherein said AG comprises an alkyl group from about 1 to 6 carbons.

12. A method according to claim 1 or 11 wherein said AG is branched, having the formula:

13. A method according to claim 12 wherein said AG is attached to said attachment linker via a (CH₂)_n group, wherein n is an integer from 0 to 4.

14. A method according to claim 12 wherein said AG is attached directly to said attachment linker.