Recombinant neospora antigens and their uses

• US 20050003433A1
• Filed: 07/26/2004
• Published: 01/06/2005
• Est. Priority Date: 03/21/1994
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Anderson, Mark L., Conrad, Patricia, Sverlow, Karen W., Barr, Bradd C.
• Granted Patent: US 7,056,501 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 33/00 Antiparasitic agents
• CPC Class Codes: A61P 33/02 Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
• CPC Class Codes: C07K 14/44 from protozoa
• CPC Class Codes: C07K 16/20 from protozoa
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6893 for protozoa
• CPC Class Codes: G01N 2469/20 Detection of antibodies in sample from host which are directed against antigens from microorganisms
• CPC Class Codes: G01N 33/56961 Plant cells or fungi

Abstract

The present invention provides isolated bovine Neospora cultures. Also provided are recombinant immunodominant Neospora antigens. The cultures and antigens are used to develop diagnostic assays for the detection of Neospora infections in cattle and other animals. Also provided are pharmaceutical compositions for the treatment and prevention of Neospora infections.

Claims

1-27. -27. (Canceled)

28. An immunological composition comprising a pharmaceutically acceptable carrier and an immunologically effective amount of bovine Neospora having all the ultrastructural and antigenic characteristics of a Neospora isolate of BPA1 (ATCC Accession No. 75710) or BPA6 (ATCC accession No. 75711).

29. The composition of claim 28, wherein the bovine Neospora is BPA1 (ATCC Accession No. 75710)

30. The composition of claim 28, wherein the bovine Neospora is BPA6 (ATCC accession No. 75711).

31. The composition of claim 28, further comprising an adjuvant.

32. The composition of claim 28, wherein the bovine Neospora is attenuated.

33. The composition of claim 32, wherein the bovine Neospora is chemically fixed.