Secreted proteins and polynucleotides encoding them

• US 20050003491A1
• Filed: 08/09/2004
• Published: 01/06/2005
• Est. Priority Date: 04/10/1997
• Status: Abandoned Application

• Current Assignee: ONO Pharmaceutical Company Limited
• Original Assignee: ONO Pharmaceutical Company Limited
• Inventors: Clark, Hilary, Collins-Racie, Lisa A., Spaulding, Vikki, Jacobs, Kenneth, McCoy, John M., LaVallie, Edward R., Agostino, Michael J., Steininger, Robert J. II, Fechtel, Kim, Merberg, David, Wong, Gordon G., Evans, Cheryl, Treacy, Maurice
• Application Number:
  US10/913,553
• Publication Number:
  US 20050003491A1
• Time in Patent Office: Days
• US Class Current: 435/69.100
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/475 Growth factors; Growth regulators
• CPC Class Codes: C07K 14/52 Cytokines; Lymphokines; Interferons
• CPC Class Codes: C07K 14/575 Hormones derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin C07K14/665, e.g. corticotropin C07K14/695
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748

Abstract

Novel polynucleotides and the proteins encoded thereby are disclosed.

Claims

1-14. -14. (Cancelled).

15. An isolated polynucleotide selected from the group consisting of:

(a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO;

173;

(b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO;

173 from nucleotide 51 to nucleotide 1202;

(c) a polynucleotide comprising the nucleotide sequence of the full-length protein coding sequence of clone lp547_—

4 deposited with the ATCC under accession number 98663;

(d) a polynucleotide encoding the full-length protein encoded by the cDNA insert of clone lp547_—

4 deposited with the ATCC under accession number 98663;

(e) a polynucleotide comprising the nucleotide sequence of the mature protein coding sequence of clone lp547_—

4 deposited with the ATCC under accession number 98663;

(f) a polynucleotide encoding the mature protein encoded by the cDNA insert of clone lp547_—

4 deposited with the ATCC under accession number 98663;

(g) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO;

174;

(h) a polynucleotide encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO;

174, the fragment comprising eight consecutive amino acids of SEQ ID NO;

174; and

(i) a polynucleotide that hybridizes under stringent conditions to any one of the polynucleotides specified in (a)-(h).

16. The polynucleotide of claim 15, wherein said polynucleotide is operably linked to at least one expression control sequence.

17. A host cell transformed with the polynucleotide of claim 16.

18. The host cell of claim 17, wherein said cell is a mammalian cell.

19. A process for producing a protein encoded by the polynucleotide of claim 16, which process comprises:

(a) growing a culture of the host cell in a suitable culture medium, wherein the host cell has been transformed with the polynucleotide of claim 16;

and

(b) purifying said protein from the culture.

20. A protein produced according to the process of claim 19.

21. An isolated polynucleotide encoding the protein of claim 20.

22. The polynucleotide of claim 21, wherein the polynucleotide comprises the cDNA insert of clone lp547_—

4 deposited with the ATCC under accession number 98663.

23. A protein comprising an amino acid sequence selected from the group consisting of:

(a) the amino acid sequence of SEQ ID NO;

174;

(b) the amino acid sequence of SEQ ID NO;

174 from amino acid 187 to amino acid 196;

(c) fragments of the amino acid sequence of SEQ ID NO;

174 comprising eight consecutive amino acids of SEQ ID NO;

174; and

(d) the amino acid sequence encoded by the cDNA insert of clone lp547_—

4 deposited with the ATCC under accession number 98663;

the protein being substantially free from other mammalian proteins.

24. The protein of claim 23, wherein said protein comprises the amino acid sequence of SEQ ID NO:

174.

25. The protein of claim 23, wherein said protein comprises the amino acid sequence of SEQ ID NO:

174 from amino acid 187 to amino acid 196.

26. A composition comprising the protein of claim 23 and a pharmaceutically acceptable carrier.