Method for the fabrication of a "lab on chip" from photoresist material for medical diagnostic applications
First Claim
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1. A method for the fabrication of a device for performing immuno assays comprising:
- (a) providing a micro structured substrate having predefined indentations and/or through holes, (b) applying at least one layer of a dry film photo resist material with functional chemical groups onto said substrate, (c) light exposing the dry film photo resist using a photo mask with a different predefined pattern, (d) developing the dry film photo resist layer, (e) repeating step (b) and step (c) using a photo mask with a different predefined pattern and step (d), so that substrate and dry film photo resist material form a capillary structure or capillary channel structure, respectively, with at least one inlet and outlet, (f) localized immobilizing biomolecules to at least one region of the inner surface of the dry film photo resist material channel structure generated in step (e) by chemical coupling via the functional groups of the dry film photo resist material, wherein a device for performing immuno assays is obtained.
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Abstract
The present invention relates to a device for performing immuno assays (“biochip” or “lab on chip” respectively), a procedure for its fabrication and the use of the device for performing immuno assays. Furthermore the present invention relates to the use of a photo lithographically patternable dry film photo resist based on a material with functional chemical groups for the immobilization of biomolecules.
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29 Claims
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1. A method for the fabrication of a device for performing immuno assays comprising:
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(a) providing a micro structured substrate having predefined indentations and/or through holes, (b) applying at least one layer of a dry film photo resist material with functional chemical groups onto said substrate, (c) light exposing the dry film photo resist using a photo mask with a different predefined pattern, (d) developing the dry film photo resist layer, (e) repeating step (b) and step (c) using a photo mask with a different predefined pattern and step (d), so that substrate and dry film photo resist material form a capillary structure or capillary channel structure, respectively, with at least one inlet and outlet, (f) localized immobilizing biomolecules to at least one region of the inner surface of the dry film photo resist material channel structure generated in step (e) by chemical coupling via the functional groups of the dry film photo resist material, wherein a device for performing immuno assays is obtained. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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- 16. A device for performing immuno assays, comprising at least one substrate and provided thereon at least one dry film photo resist polymer material layer comprising functional chemical groups, wherein substrate and dry film photo resist polymer material together form a capillary structure with at least one inlet and one outlet, wherein biomolecules are bound to at least one region of the inner surface of the capillary structure, formed by the dry film photo resist material, by a chemical link via the functional groups of the dry film photo resist material.
- 26. Use of a photo lithographically patternable dry film photo resist based on a material with functional chemical groups for the immobilization of biomolecules.
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