Ectocornea-like sheet and method of constructing the same
First Claim
Patent Images
1. A corneal epithelium-like sheet, comprising a cell layer in which a layered structure of cells derived from oral mucosal epithelial cells is formed.
2 Assignments
0 Petitions
Accused Products
Abstract
It is intended to provide a transplantation material applicable to ocular surface diseases with a need for ectocornea transplantation (i.e., an ectocornea-like sheet). Oral mucosal epithelial cells are inoculated onto an amnion and then cultured in the coexistence of supporter cells. When a layered structure of the oral mucosal epithelial cells is formed, the outermost layer is brought into contact with air, thereby inducing differentiation. Thus, an ectocornea-like sheet having an oral mucosal epithelial cell layer on the amnion is obtained.
11 Citations
20 Claims
- 1. A corneal epithelium-like sheet, comprising a cell layer in which a layered structure of cells derived from oral mucosal epithelial cells is formed.
- 5. A corneal epithelium transplantation sheet, comprising a cell layer in which a layered structure of cells derived from oral mucosal epithelial cells is formed on a collagen layer.
-
11. A method of constructing a corneal epithelium-like sheet, the method comprising:
-
a) culturing oral mucosal epithelial cells on a collagen layer; and
b) when the oral mucosal epithelial cells are proliferated and a layered structure of the cells is formed, bringing the outermost layer into contact with the air. - View Dependent Claims (12, 13, 14, 15)
-
-
16. A method of constructing a corneal epithelium-like sheet, comprising the steps of:
-
inoculating supporter cells in a first container to form a supporter cell layer;
setting a second container, which has a bottom face made of an isolation membrane with pore size through which the supporter cells cannot pass, in the first container so that the bottom face is located in a culture medium;
forming a collagen layer on the bottom face of the second container;
inoculating oral mucosal epithelial cells on the collagen layer;
culturing the oral mucosal epithelial cells to form a layered structure of the cells; and
bringing the outermost layer of the layered structure of the oral mucosal epithelial cells into contact with the air. - View Dependent Claims (17, 18)
-
Specification