Delta4-desaturase genes and uses thereof
First Claim
1. An isolated nucleotide sequence or fragment thereof comprising or complementary to a nucleotide sequence encoding a polypeptide having desaturase activity, wherein the amino acid sequence of said polypeptide has at least 90% identity to an amino acid sequence comprising SEQ ID NO:
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Abstract
The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 4 (i.e., “Δ4-desaturase”). In particular, Δ4-desaturase may be utilized, for example, in the conversion of adrenic acid to ω6-docosapentaenoic acid and in the conversion of ω3-docosapentaenoic acid to docosahexaenoic acid. The polyunsaturated fatty acids produced by use of the enzyme may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
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Citations
15 Claims
- 1. An isolated nucleotide sequence or fragment thereof comprising or complementary to a nucleotide sequence encoding a polypeptide having desaturase activity, wherein the amino acid sequence of said polypeptide has at least 90% identity to an amino acid sequence comprising SEQ ID NO:
- 2. An isolated nucleotide sequence or fragment thereof comprising or complementary to a nucleotide sequence having at least 90% identity to a nucleotide sequence comprising SEQ ID NO:
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3. (cancelled)
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4. (cancelled)
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7. (cancelled)
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8. A method of producing a desaturase comprising the steps of:
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a) isolating a nucleotide sequence comprising or complementary to a nucleotide sequence;
i) encoding a polypeptide comprising an amino acid sequence having at least 90% identity to an amino acid sequence comprising SEQ ID NO;
20 orii) having at least 90% identity to a nucleotide sequence comprising SEQ ID NO;
16;
b) constructing a vector comprising;
i) said isolated nucleotide sequence operably linked to ii) a promoter; and
c) introducing said vector into a host cell for a time and under conditions sufficient for expression of said desaturase.
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9. A vector comprising:
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a) an isolated nucleotide sequence comprising or complementary to a nucleotide sequence;
i) encoding a polypeptide having comprising an amino acid sequence having at least 90% identity to an amino acid sequence comprising SEQ ID NO;
20 orii) having at least 90% identity to a nucleotide sequence comprising SEQ ID NO;
16, operably linked tob) a promoter. - View Dependent Claims (10, 11, 12, 14)
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13. (cancelled)
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15-31. -31. (cancelled)
Specification