Method for the devitalisation of natural organs and/or for the preparation of extracellular matrices for tissue engineering
First Claim
1. Procedure or process for devitalization and preservation of organs and/or tissues, comprising the following steps:
- a. sterile harvest and storage of the organ or the tissue in a liquid selected from the group consisting of;
sterile water, a crystalloid liquid, a colloidal liquid, a lipid-containing liquid or a combination of the mentioned liquids, until the devitalization is achieved, b. the outwash of cell-fragments, cellular decomposition products (debris) as well as soluble substances under pressure with a liquid, selected from the group consisting of;
sterile water, a crystalloid liquid, a colloidal liquid, a lipid-containing liquid and a combination of the mentioned liquids.
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Abstract
74. Culture medium according to any one of claims 48 to 73, characterized in that, that autologous growth factors are gained through chemical and/or biochemical destruction of body-own tissues.
75. Culture medium according to any one of claims 48 to 74, characterized in that, that autologous growth factors are gained through apoptosis of body-own tissues.
76. Culture medium according to claim 74, characterized by the fact that tissue destruction is done by ultrasound. Summary The invention provides new procedures for the devitalization and preserving of human and animal organs and tissues, preferably however natural hollow organs and all of their components, in particular from blood vessels and cardiac valves. Furthermore the invention provides procedures for the production of matrices for construction of organs and tissues in part or in toto. In addition the invention concerns organs and tissues, in particular natural and artificial hollow organs which can be achieved according to invention-appropriate procedures. Furthermore the invention concerns the clinical use of these organs and tissues an the application in the human and veterinarian medicine, preferably in the cardiac and vascular surgery. Furthermore the invention concerns new culture media.
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Citations
73 Claims
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1. Procedure or process for devitalization and preservation of organs and/or tissues, comprising the following steps:
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a. sterile harvest and storage of the organ or the tissue in a liquid selected from the group consisting of;
sterile water, a crystalloid liquid, a colloidal liquid, a lipid-containing liquid or a combination of the mentioned liquids, until the devitalization is achieved,b. the outwash of cell-fragments, cellular decomposition products (debris) as well as soluble substances under pressure with a liquid, selected from the group consisting of;
sterile water, a crystalloid liquid, a colloidal liquid, a lipid-containing liquid and a combination of the mentioned liquids. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 69)
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32. The procedure or process of caim 30 wherein the hollow organs are selected from the group consisting of:
- allogenic vessels, to xenogenic vessels and ureter.
- 48. Culture medium for the increase of growth, remodeling processes and reduction of dedifferenziation of vascular cells in tissue culture, characterized in that autologous growth factors and/or adhesion molecules are added to a basal chemically defined medium or to a full medium.
Specification