Apparatus including ion transport detecting structures and methods of use
First Claim
1. A biochip comprising:
- a) at least one ion transport measuring means;
b) at least one pressure generating structure comprising at least one fluidic channel connected to said at least one ion transport measuring means, wherein at least a portion of the surface of said at least one fluidic channel is electrically charged such that when said at least one fluidic channel contains a solution and an appropriate electrical field is established in said at least one fluidic channel, pressure is produced by electroosmotic flow near said ion transport measuring means that can transport a particle toward, at, on, or near said at least one ion transport measuring means.
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Accused Products
Abstract
The present invention recognizes that the determination of ion transport function or properties using direct detection methods, such as whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and other fluidic components and methods of use that allow for the direct analysis of ion transport function or properties using microfabricated structures that can allow for automated detection of ion transport function or properties. These biochips and fluidic components and methods of use thereof are particularly appropriate for automating the detection of ion transport function or properties, particularly for screening purposes.
187 Citations
35 Claims
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1. A biochip comprising:
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a) at least one ion transport measuring means;
b) at least one pressure generating structure comprising at least one fluidic channel connected to said at least one ion transport measuring means, wherein at least a portion of the surface of said at least one fluidic channel is electrically charged such that when said at least one fluidic channel contains a solution and an appropriate electrical field is established in said at least one fluidic channel, pressure is produced by electroosmotic flow near said ion transport measuring means that can transport a particle toward, at, on, or near said at least one ion transport measuring means. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A biochip comprising an array of ion transport measuring recording units,
wherein each of said ion transport measuring recording units comprises a hole that extends through said biochip, and at least one particle positioning means, wherein said hole is made at least in part by laser ablation.
- 14. A fluidic component comprising a tube, wherein the wall of said tube comprises one or more holes having a diameter of less than about 10 microns.
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26. A chip comprising an ion transport measuring means that comprises:
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at least one counter pore;
at least one hole connected to said at least one counter pore;
wherein together said at least one counter pore and said at least one hole allow fluid communication between the upper surface and the lower surface of said chip. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35)
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Specification