Recombinational cloning using nucleic acids having recombination sites

• US 20050009091A1
• Filed: 08/19/2004
• Published: 01/13/2005
• Est. Priority Date: 06/07/1995
• Status: Abandoned Application

• Current Assignee: Invitrogen Corp. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Invitrogen Corp. (Thermo Fisher Scientific Incorporated)
• Inventors: Fox, Donna K., Temple, Gary F., Hartley, James L., Brasch, Michael A.
• Application Number:
  US10/921,265
• Publication Number:
  US 20050009091A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12N 15/10 Processes for the isolation, preparation or purification of DNA or RNA chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34
• CPC Class Codes: C12N 15/64 General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
• CPC Class Codes: C12N 15/66 General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
• CPC Class Codes: C12N 9/00 Enzymes; Proenzymes; Compositions thereof preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113; Processes for preparing, activating, inhibiting, separating or purifying enzymes preparation of malt C12C1/00

Abstract

Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

Claims

1. (Canceled).

2. A nucleic acid molecule comprising at least one nucleotide sequence that has at least 80-99% homology to a nucleotide sequence selected from the group of sequences consisting of:

4. A nucleic acid molecule that hybridizes to the nucleic acid molecule of claim 2.

6. The nucleic acid molecule of any one of claims 2-5, wherein said nucleic acid molecule is a vector.

7. The nucleic acid molecule of any one of claims 2-5, wherein said nucleic acid molecule is a primer molecule.

8. The nucleic acid molecule of any one of claims 2-5, wherein said nucleic acid molecule is contained within a host cell.

9. The nucleic acid molecule of any one of claims 2-5, wherein said nucleic acid molecule is an isolated molecule.

3. A nucleic acid molecule comprising at least one nucleotide sequence that has at least 80-99% homology to a nucleotide sequence selected from the group of sequences consisting of:

5. A nucleic acid molecule that hybridizes to the nucleic acid molecule of claim 3.